BRAP

• Plasmid: pGEX6P HALO Thr-BRAP
• Parent Plasmid: pGEX 3C HALO Thr
• Expressed: GST-HALO-BRAP
• Genbank: NP_006759.3 (uni: Q7Z569-1)
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

ggatccATGAGTGTGTCACTGGTTGTTATCCGATTGGAGCTCGCGGAACA
CTCGCCTGTCCCCGCCGGCTTCGGCTTCAGCGCCGCGGCCGGGGAAATGT
CTGATGAGGAGATAAAAAAGACGACACTAGCCTCAGCTGTAGCCTGTTTA
GAAGGCAAGTCACCAGGAGAGAAAGTAGCGATTATCCATCAGCATCTCGG
CCGTCGAGAAATGACAGATGTGATCATTGAGACCATGAAGTCCAACCCAG
ATGAACTAAAAACTACAGTGGAAGAAAGGAAGTCTTCAGAAGCCTCCCCC
ACTGCGCAAAGAAGTAAAGATCACAGTAAGGAATGCATAAACGCTGCCCC
AGATTCTCCGTCCAAACAGCTTCCAGACCAGATTTCATTCTTCAGTGGAA
ATCCATCAGTTGAAATAGTTCATGGTATTATGCACCTATATAAGACAAAT
AAGATGACCTCCTTAAAAGAAGATGTGCGGCGCAGTGCCATGCTGTGTAT
TCTCACAGTCCCTGCTGCAATGACCAGTCATGACCTTATGAAGTTTGTTG
CCCCATTTAACGAAGTAATTGAACAAATGAAAATTATCAGAGACTCTACT
CCCAACCAATATATGGTGCTGATAAAGTTTCGTGCACAGGCTGATGCGGA
TAGTTTTTATATGACATGCAATGGCCGCCAGTTCAACTCAATAGAAGATG
ACGTTTGCCAGCTAGTGTATGTGGAAAGAGCTGAAGTGCTCAAATCTGAA
GATGGCGCCAGCCTCCCAGTGATGGACCTGACTGAACTCCCCAAGTGCAC
GGTGTGTCTGGAGCGCATGGACGAGTCTGTGAATGGCATCCTCACAACGT
TATGTAACCACAGCTTCCACAGCCAGTGTCTACAGCGCTGGGACGATACC
ACGTGTCCTGTTTGCCGGTACTGTCAAACGCCCGAGCCAGTAGAAGAAAA
TAAGTGTTTTGAGTGTGGTGTTCAGGAAAATCTTTGGATTTGTTTAATAT
GCGGCCACATAGGATGTGGACGGTATGTCAGTCGACATGCTTATAAGCAC
TTTGAGGAAACGCAGCACACGTATGCCATGCAGCTTACCAACCATCGAGT
CTGGGACTATGCTGGAGATAACTATGTTCATCGACTGGTTGCAAGTAAAA
CAGATGGAAAAATAGTACAGTATGAATGTGAGGGGGATACTTGCCAGGAA
GAGAAAATAGATGCCTTACAGTTAGAGTATTCATATTTACTAACAAGCCA
GCTGGAATCTCAGCGAATCTACTGGGAAAACAAGATAGTTCGGATAGAGA
AGGACACAGCAGAGGAAATTAACAACATGAAGACCAAGTTTAAAGAAACA
ATTGAGAAGTGTGATAATCTAGAGCACAAACTAAATGATCTCCTAAAAGA
AAAGCAGTCTGTGGAAAGAAAGTGCACTCAGCTAAACACAAAAGTGGCCA
AACTCACCAACGAGCTCAAAGAGGAGCAGGAAATGAACAAGTGTTTGCGA
GCCAACCAAGTCCTCCTGCAGAACAAGCTAAAAGAGGAGGAGAGGGTGCT
GAAGGAGACCTGTGACCAAAAAGATCTGCAGATCACCGAGATCCAGGAGC
AGCTGCGTGACGTCATGTTCTACCTGGAGACACAGCAGAAGATCAACCAT
CTGCCTGCCGAGACCCGGCAGGAAATCCAGGAGGGACAGATCAACATCGC
CATGGCCTCGGCCTCGAGCCCTGCCTCTTCGGGGGGCAGTGGGAAGTTGC
CCTCCAGGAAGGGCCGCAGCAAGAGGGGCAAGTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMSVSLVVIRLELAE
HSPVPAGFGFSAAAGEMSDEEIKKTTLASAVACLEGKSPGEKVAIIHQHL
GRREMTDVIIETMKSNPDELKTTVEERKSSEASPTAQRSKDHSKECINAA
PDSPSKQLPDQISFFSGNPSVEIVHGIMHLYKTNKMTSLKEDVRRSAMLC
ILTVPAAMTSHDLMKFVAPFNEVIEQMKIIRDSTPNQYMVLIKFRAQADA
DSFYMTCNGRQFNSIEDDVCQLVYVERAEVLKSEDGASLPVMDLTELPKC
TVCLERMDESVNGILTTLCNHSFHSQCLQRWDDTTCPVCRYCQTPEPVEE
NKCFECGVQENLWICLICGHIGCGRYVSRHAYKHFEETQHTYAMQLTNHR
VWDYAGDNYVHRLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTS
QLESQRIYWENKIVRIEKDTAEEINNMKTKFKETIEKCDNLEHKLNDLLK
EKQSVERKCTQLNTKVAKLTNELKEEQEMNKCLRANQVLLQNKLKEEERV
LKETCDQKDLQITEIQEQLRDVMFYLETQQKINHLPAETRQEIQEGQINI
AMASASSPASSGGSGKLPSRKGRSKRGK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28603
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee