FancI (xenopus)

• Plasmid: pFBDM6P1b 6His TEV HA xenopus fancd2/V5 xenopus fanci
• Parent Plasmid: pFBDM6P1b
• Expressed: V5 xenopus fanci
• Genbank: NM_001171211.1 GI:284005540
• Species: Xenopus laevis

Sequence of Insert

CTCGAGGAAACCATGGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGATGGACCAAAAAATTCTGTCTTTGGCTGCTGAAGAACAAAATGATGGACTTCAGAGTTATCTTCAAAACCTGAAAGAAACCGAGCTTTCAGAAATCATAACAAAGCATGCAGTTAAGGGAAAAGACTGTGGAGCGCTATTGCGAGGCGTCTTTAAAGGGTCTCCGTGCTCTCATGATGTAGCCGTACGCCGAAGGCTCGCGGTTTATAGACACTGCATACAGCTTGTTGAGTCCGGGGACTTGCAACGAGAGGTTGCATCTGAGATTATGGGTTTATTAATGCTGGAGGTCCATCATTTTCCGGGTGCTTCGCTCATTGAACTGGCTAACTTATTTGCGGATGCTGTTAAAGCTGGTACTCTGTCCAATGGAAAATCTTTAGATTTGTTTCCCACAGTACTTACTGCGCTTTCTTCAAAAGAAAGCTTAGCATATGGTAAAGGTGAACTTACTGGAGAGGAATTTAAGAAACAACTTATAAATAGCCTTTGTTCTAGCAGATGGGATCCACAGAGTGTGATTTTTCTTACTTCAATGTTTAGGGATGTTTCCTTGAGTGCCGAAGAGCTGCAGTTTGTTGTGGAAAAGGTTTTAAGGATGCTTTCTAAGTTAGACCTCCAGGAAATGCCCCCCTTAGTTTACCAATTGCTACTCCTGTCTGCTAAGGGTAGTAAAAAGAATATTGTGGAAGGAATTATCACTATATTTAATGATCTTGATCAGAAGCAGTTAGTACAGCAAGAGAACTCTGAATCATTAGACTTGGAAGATGCAACCATCCCACAAGACCAGCTGCACCACGTGGAGGGCACAATCATTTTACACATCGTTTTTGCAATTAAGTTGGACCAGGAATTAGGAAGAGAGCTTCTGAAGTATCTCAAGGCTGGCCAACAAGGGGACTCAAGCAAGATCCTTTGCCCTTTCAGTGCTGCCCTCTTGTTATCTGTATCAAGGATTCATAGATTCCAAGAACAGGTATTTGATTTTCTCAAATCTACTATACTGAGAGGTTATAAAGACCTTCAGTTTCATCAAAGCTCAAAATTCCTTCAGGACTTAGTTCCCCAGCCTGTATGCGTATCTTCAATTCTTCTGGAAACTGTTAAAAACAGTGTTTATGGATGGGACCATGTCACTCAAGGGCTTGTGGAACTTGGTTTTATTTTAATGGATTCTTTTGGACCCAAAGCAGCCTTTGGAAATAAAGTGGTTGAGCTTATTTCCGTGCATTCCAGGACACCTTCCCAGCAAGCTTGTCAGCTTGGATCTAGAATCTTGCTAGAAACTTTTAAGGTCCATGAGCCTATCAGAAGTGAAATTCTGGAGCAGGTCTTGAATCGTGTTATCACAAAAGCTGCAACTCCAGTTACACATTTTATAGATCTTTTATCAGATATTGTGGTTTCTGCTCCGCTCATCCTTCAGAATTCATCATCTAAAGTTACTGAAGCATTTGACCACTTGTCCTTCCTGCCTCTTACAACTGTTCAGGGGCTCCTGAAAGCTGTGCAGCCCCTTCTTAAAATCAGTATGTCCATGAGGGATTCCCTTATTCTTGTTCTAAGAAAAGCCATGTTCTCAAGTCAAATAGATGCAAGAAAGTCGGCTGTCGCTGGGTTTCTTCTCCTACTGAGGAACTTTAAAGTCTTAGGAAGTCTGTCCTCATCTCAGTGCAGCCAAGCTATTGGTGCTAGCCAGATCCAGGTGGATGTCCATATGCGTTACAATGCTGCAGCAAATGAGGCCTTTTGTCTGGAAATCCTTGGCAGTCTGAGGAGGTGCTTAAGCCAGCAAGCAGATGTTAGGCTCATGCTTTACGAGGGTTTCTATGATGTCCTGCGACGAAATTCCCAGCTTGCCAGTTCTGTCATGCAGACTCTGTTGTCTCAGTTAAAGCGATATTATGAACCCGAACCTGATCTTTTGCCACCATTGAAGTTGGAGGGCTGTATTACTGCTCAAGGGGATCATATATTTTTGCAGGAACCTCTGGCTCACCTGTTGTGCTGTATCCACCATTGCTTGCAGTGGTATAAGAGCAGTTTACAGCAGCATAGGAATCCTGATGAAGATGATGACGATGATGACCAAACGGGATGTCAACAGGACCTTAATGATATAATGGAGTCCATCACAAGGAGAATGATTAAATGTGACCTGGAGGACTTTGAATTAGACAAGTCTGCAGACTTTTCTTTGGCGTCTGGTGTTGGAGTGAAAAATAACATATATGCTGTTTTGGTAATGGGGATTTGTGAAGTCCTAATAGAATACAACTTTATCATTGCAAATTTCAGCAAGAGCAAATTTGAGGACATTTTGGGCCTGTTTAAGTGCTACAGCAAACTGTCCGATATCTTAAAGGAGAAAGCTGCTAAAGGCAGACAGCCTGGGAACAATAAAACGGCCAGAAGCCTCATATCAATGACTTTTGTTTCTACACTCTTAACTGCTTTATTCAGAGACAGCACACGCTCGCACGAGGAGAGTCTTTCTATTCTTCGCGCAAATGTGGATTTCATGCGCTATTCGGTGTGTGTTGCCCTTCAAAAAATTCAGCAGCTTGAAGAAACTGGTGTCACAGATGGACCTGATGGACAGAACAGCGAGAAAATGTTCCGAAGTCTATGTGAAATTACCAGGGTACTTATGTGGAGATACACCTCAATTCCAGCTGCAGCTGAGAATCCTGGGAAGAAGGACAAAGGCAAAACCATTTCCTTGCTGTGCCTGGAGGGATTGCTGAGAGTGTTTAACACCGTTCAGCAACGATATCCATCAAAGATCCCACAATTCCTTACTGCGTTGGATGCCCTAGGGGATGAGGATGAAGAAGGCTCAAGAGAAATCAATGTGACCGAGAAGGCAGCTTTTCAGATTAAACAGTTTCAAAGATCTCTTATTAACCAACTCAGTGGTGGAGAGGATGACTTTAACAGCAAAGAAGCTCTACTGTTAGTCAGCATCTTGTCTACATTATCCAGGTTACTGGCCCCCTCTTCTCAACAGTTCGTGCAGATGCTGTCTTGGACTGTTAAAATCTGTAAAGAGACAAACATTGAGGATGTCCAGTTTTGCAAGGGAATAATGAACCTTCTCTTTAGCCTCCATGTTCAGTTCAAGAGCCCAGTGAGTGTGCTTCGAGAACTGTGCCAGGACATCCATGGGCATCTGGGTGATATTGATCAGGATATTGAAGTGGAGAAGCAGTCTCATTTTGCTACTGTAAGTCTCAAGACTGTAGCCCCGACTGTTACTTTGTTGGTTCTGGGTCAAGCTGCAAAGGTCCTGGAAGAAGTGGACTGGCTGATCATAAAGCTGAAAGGACTACTGGGTTCAGAGAAGCTCTCTACGGAAGATTTAACTCAGACTTCAAATGCAAGGGTTCCAATAGAAAAAGCTATCATTCTTCAGTTGGGCACACTGCTGACTGCCTGCCATGAGTTAGTGCAGACTGCTCTACCTGCTGGGAGTTGTACTGACACTCTTCTGAAGGAGCTGGCAAAGATGTACACCATCCTCACGTCCGTTGTCAAATACTACTTACAGATCTGCAGCAGTCACGGGGGACAGATTTCTGCTCGGTTGGAGAAGCTGGTGAAGCTTTCGGGTTCTCATCTTACCCCACAATGTTATGCCTTCATCACATATGTACAGAACATCCACAGTGAAAGTCTAAGCCTTGCTGCAGAAAAGAAGAAGAAAAAGAAGGAAGAGGCATTGACTGCCACCTCAGCCAAAATCCTGCGTGATACTAAGCCCATACCAAACCTGATCTTTGCCATTGAACAGTATGAGAAGTTTCTCATTCATCTCTCCAAGAAGTCCAAGGTGAACTTGATGCAATACATGAAGCTGAGCACCTCAAGGGACTTCAGGATAAATGCAGCCACACTAGACGCAGCTTTACAAGAGAAAGGGTCTGAAGATGAAGAGAATGAGCCAGATAATGAACAGGCTGTAACAGAAGAAGAAAGTCAAGAACCAAAGAAGAAGAGGCGCAGAAAATAACTCGAG

Amino Acid Sequence

MGKPIPNPLLGLDSTMDQKILSLAAEEQNDGLQSYLQNLKETELSEIITKHAVKGKDCGALLRGVFKGSPCSHDVAVRRRLAVYRHCIQLVESGDLQREV
ASEIMGLLMLEVHHFPGASLIELANLFADAVKAGTLSNGKSLDLFPTVLTALSSKESLAYGKGELTGEEFKKQLINSLCSSRWDPQSVIFLTSMFRDVSL
SAEELQFVVEKVLRMLSKLDLQEMPPLVYQLLLLSAKGSKKNIVEGIITIFNDLDQKQLVQQENSESLDLEDATIPQDQLHHVEGTIILHIVFAIKLDQE
LGRELLKYLKAGQQGDSSKILCPFSAALLLSVSRIHRFQEQVFDFLKSTILRGYKDLQFHQSSKFLQDLVPQPVCVSSILLETVKNSVYGWDHVTQGLVE
LGFILMDSFGPKAAFGNKVVELISVHSRTPSQQACQLGSRILLETFKVHEPIRSEILEQVLNRVITKAATPVTHFIDLLSDIVVSAPLILQNSSSKVTEA
FDHLSFLPLTTVQGLLKAVQPLLKISMSMRDSLILVLRKAMFSSQIDARKSAVAGFLLLLRNFKVLGSLSSSQCSQAIGASQIQVDVHMRYNAAANEAFC
LEILGSLRRCLSQQADVRLMLYEGFYDVLRRNSQLASSVMQTLLSQLKRYYEPEPDLLPPLKLEGCITAQGDHIFLQEPLAHLLCCIHHCLQWYKSSLQQ
HRNPDEDDDDDDQTGCQQDLNDIMESITRRMIKCDLEDFELDKSADFSLASGVGVKNNIYAVLVMGICEVLIEYNFIIANFSKSKFEDILGLFKCYSKLS
DILKEKAAKGRQPGNNKTARSLISMTFVSTLLTALFRDSTRSHEESLSILRANVDFMRYSVCVALQKIQQLEETGVTDGPDGQNSEKMFRSLCEITRVLM
WRYTSIPAAAENPGKKDKGKTISLLCLEGLLRVFNTVQQRYPSKIPQFLTALDALGDEDEEGSREINVTEKAAFQIKQFQRSLINQLSGGEDDFNSKEAL
LLVSILSTLSRLLAPSSQQFVQMLSWTVKICKETNIEDVQFCKGIMNLLFSLHVQFKSPVSVLRELCQDIHGHLGDIDQDIEVEKQSHFATVSLKTVAPT
VTLLVLGQAAKVLEEVDWLIIKLKGLLGSEKLSTEDLTQTSNARVPIEKAIILQLGTLLTACHELVQTALPAGSCTDTLLKELAKMYTILTSVVKYYLQI
CSSHGGQISARLEKLVKLSGSHLTPQCYAFITYVQNIHSESLSLAAEKKKKKKEEALTATSAKILRDTKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQYM
KLSTSRDFRINAATLDAALQEKGSEDEENEPDNEQAVTEEESQEPKKKRRRK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU53172
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee