Rnf12 (Mouse)

• Synonyms: AL022832; AW743871; Ha1r; Rlim
• Plasmid: pCAGGS puro 3XFLAG mouse Rnf12 H569A C572A
• Parent Plasmid: pCAGGS puro 3FLAG
• Expressed: 3XFLAG-mouse Rnf12 H569A C572A
• Genbank: NM_011276.3 GI:47078288
• Tag: 3FLAG
• Species: Mouse

Sequence of Insert

GGATCCATGGAGAACTCAGATTCTAACGATAAAGGAAGTGACCAGTCTGCAGCTCAGCGCAGAAGTCAAATGGATCGCTTGGATCGGGAAGAGGCTTTCTATCAATTTGTGAATAATCTCAGTGAAGAAGATTATAGACTTATGAGAGACAACAATTTGCTGGGCACCCCAGGTGAAAGTACTGAGGAAGAGTTGCTCAGAAGACTACAACAAATTAAAGAGGGTCCACCACCACAGAGCCCCGATGAAAATAGAGCTGGAGAGTCTTCAGATGATGTGACCAACAGTGATTCTATAATAGATTGGCTTAACTCAGTCAGACAGACTGGAAATACAACAAGAAGTGGTCAGAGAGGAAACCAGTCCTGGAGAGCAGTGAGCCGGACTAATCCAAACAGCGGTGATTTCAGATTCAGTTTAGAGATAAATGTTAACCGTAACAATGGAAGCCAAACTTCCGAGAATGAAAGTGAACCATCCACTAGGCGTCTTAGTGTAGAAAACATGGAGAGCAGCAGCCAAAGGCAAATGGAAAATTCTGCATCTGAATCAGCATCGGCCAGACCTTCTCGAGCGGAGCGCAATTCAGCTGAAGCAGTAACAGAAGTGCCGACCACCAGAGCTCAGAGGAGGGCAAGAAGCCGGAGCCCAGAGCATCGGAGAACCAGAGCAAGAGCTGAAAGAAGCAGGTCCCCTCTGCAGCCAACAAGTGAAATTCCACGAAGAGCTCCGACCTTGGAGCAGTCTTCGGAAAACGAGCCTGAAGGAAGTTCTAGAACCCGGCACCATGTGACCCTGAGACAGCAGATATCTGGACCGGAGTTGTTAGGTAGAGGTCTTTTTGCAGCTTCTGGATCAAGAAACCCCTCTCAGGGGACAAGCTCTTCAGACACAGGTAGCAATAGTGAATCCTCAGGATCTGGCCAAAGACCTCCAACCATAGTTCTTGATCTTCAAGTAAGAAGAGTTCGTCCTGGAGAATACCGGCAGAGAGATAGTATAGCTAGCAGAACTCGATCAAGATCTCAGGCTCCGAACAACACAGTTACTTATGAAAGTGAACGTGGAGGTTTTAGGCGTACCTTTTCCCGTTCTGAACGTGCAGGTGTGAGAACCTATGTGAGTACTATCAGGATTCCTATTCGCAGAATCTTAAATACTGGATTAAGTGAAACTACATCTGTTGCAATTCAAACCATGTTAAGGCAGATAATGACAGGCTTTGGTGAGCTAAGCTACTTTATGTACAGTGACAGTGATTCAGAGCCTAGTGCCTCAGTCTCCAGCCGAAATGTGGAGAGAGTAGAGTCACGGAATGGAAGAGGGAGCTCTGGTGGCGGTAACAGCTCTGGTTCCAGTTCCAGCTCAAGTCCTAGCCCCAGTTCAAGTGGTGAAAGTTCCGAGAGTAGCTCAGAGATGTTCGAAGGCAGTAGTGAAGGAGGCTCGTCTGGCCCATCCAGAAGAGATGGTCGACATAGGGCCCCAGTCACATTTGATGAAAGTGGCTCTCTGCCATTCCTTAGTCTGGCTCAGTTTTTTCTTTTAAATGAAGATGATGAAGACCAACCTAGAGGACTCACTAAAGAACAGATCGATAACTTGGCAATGAGAAGCTTTGGTGAAAATGATGCATTGAAAACGTGTAGTGTTTGCATTACAGAATACACAGAAGGCAACAAACTTCGTAAACTACCTTGCTCCCATGAGTACGCCGTCCACGCCATCGACCGCTGGTTGTCTGAGAATTCCACCTGTCCCATTTGTCGCAGGGCAGTCTTATCTTCTGGGAACAGAGAAAGTGTTGTGTGAGCGGCCGC

Amino Acid Sequence

MDYKDHDGDYKDHDIDYKDDDDKGSMENSDSNDKGSDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQIKEGPP
PQSPDENRAGESSDDVTNSDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRNNGSQTSENESEPSTRRLSVENMESSSQRQM
ENSASESASARPSRAERNSAEAVTEVPTTRAQRRARSRSPEHRRTRARAERSRSPLQPTSEIPRRAPTLEQSSENEPEGSSRTRHHVTLRQQISGPELLG
RGLFAASGSRNPSQGTSSSDTGSNSESSGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQAPNNTVTYESERGGFRRTFSRSERAGVRTYVSTI
RIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYSDSDSEPSASVSSRNVERVESRNGRGSSGGGNSSGSSSSSSPSPSSSGESSESSSEMFEGS
SEGGSSGPSRRDGRHRAPVTFDESGSLPFLSLAQFFLLNEDDEDQPRGLTKEQIDNLAMRSFGENDALKTCSVCITEYTEGNKLRKLPCSHEYAVHAIDR
WLSENSTCPICRRAVLSSGNRESVV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU50604
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee