PINK1 (Tribolium) Codon optimised sequence

• Plasmid: pET6HIS SUMO Pink1 tribolium 150-570 S205D D359A E527A K528A
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO Pink1 tribolium 150-570 S205D D359A E527A K528A
• Tag: 6His
• Tag: SUMO
• Species: Tribolium castaneum

Sequence of Insert

AGCCGCTTTGAATCTAACCCGATCACCCTGAATGATCTGAGTCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAG
ATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTCAACTACGATATTCAGGACAATTCTATGGAAATCCTGAAAGCTATGTATCG
TGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAAT
ATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAG
GTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCT
GCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAA
CCGGAATCGCCGATTCTGGTTATCTCGGCTTTTGGCTGCTGTCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAG
GCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGC
AATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGAC
GAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGT
TTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTG
CGAAGGCAAAATCAACAACAAATCCTTCGGTGCAGCATTCACCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGC
GCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCTGCCGGAACTGGATTAAGCGGCCGCGTAAAGATCCGGCTGCTAACAAAGCC
CGAAAGAGGCTGAATGG

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKMMFNYDIQDNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLP
PHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLL
DTSEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLP
KLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGAAFTRNWRRTYPEYLLISS
FLCRAKLANVRNALHWIQENLPELD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51142
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee