RFWD3

• Synonyms: RNF201
• Plasmid: pMEX3C RFWD3 I289D
• Parent Plasmid: pMEX3C
• Expressed: MBP-RFWD3 I289D
• Genbank: NM_018124.3 GI:71143111
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGGCTCATGAAGCAATGGAATATGATGTTCAGGTGCAGTTAAATCATGCCGAACAACAGCCAGCTCCTGCTGGCATGGCCAGCAGCCAAGGGGGACCAGCCCTCCTCCAGCCTGTTCCTGCTGATGTGGTCAGCAGCCAGGGGGTACCATCCATCCTCCAGCCAGCTCCTGCTGAGGTGATCAGCAGCCAAGCGACACCACCCCTGCTCCAGCCTGCTCCGCAACTGTCTGTTGACCTGACAGAAGTGGAGGTCTTGGGAGAAGACACTGTGGAGAACATCAATCCAAGAACTTCAGAACAACATAGGCAGGGATCTGATGGTAATCACACCATCCCAGCATCTTCGTTGCATTCAATGACCAACTTCATCAGCGGACTGCAGAGACTTCATGGCATGCTGGAATTCCTGAGACCTTCATCTTCAAACCACAGTGTAGGGCCAATGAGAACAAGAAGGAGGGTATCTGCTTCACGGAGGGCAAGAGCCGGAGGGTCTCAGAGGACAGACAGTGCCAGGTTGAGAGCACCATTGGATGCTTACTTTCAGGTGAGCAGGACCCAGCCTGACTTGCCAGCTACCACTTATGATTCAGAGACTAGGAATCCTGTATCTGAAGAGTTGCAGGTGTCTAGTAGTTCTGATTCTGACAGTGACAGCTCTGCAGAGTATGGAGGGGTTGTTGACCAGGCAGAGGAATCTGGAGCTGTCATTTTAGAAGAGCAACTAGCAGGTGTCTCAGCAGAGCAAGAAGTTACATGTATCGATGGAGGCAAGACCCTCCCCAAACAGCCATCTCCCCAGAAGTCTGAGCCTCTGCTACCTTCTGCTTCTATGGATGAGGAAGAAGGGGACACTTGTACAGACTGTCTGGAACAGTGGACCAATGCTGGGGACCACCGGCTCTCAGCATTACGCTGTGGGCATCTCTTTGGGTATAGGTGCATTTCCACGTGGCTTAAAGGACAAGTACGAAAATGTCCCCAGTGCAACAAGAAAGCCAGGCACAGTGACATTGTCGTCCTTTATGCCCGAACCCTGAGAGCTTTGGACACTAGTGAACAGGAGCGCATGAAAAGTTCCCTACTGAAGGAACAGATGCTAAGGAAACAGGCCGAGTTAGAATCAGCACAGTGCCGACTCCAACTGCAGGTCCTCACTGATAAGTGCACTAGGCTTCAAAGGCGTGTTCAGGACTTGCAAAAACTTACGTCACATCAAAGTCAGAATTTACAGCAACCCAGGGGCTCCCAAGCATGGGTCCTGAGCTGCTCACCCTCCAGCCAGGGCCAGCACAAGCACAAGTACCACTTCCAAAAGACCTTCACAGTATCTCAGGCAGGAAACTGCCGGATCATGGCATACTGTGATGCTCTGAGCTGCCTGGTGATATCACAGCCTTCTCCTCAGGCCTCTTTTCTTCCAGGCTTTGGTGTTAAGATGTTGAGTACTGCCAACATGAAGAGCAGTCAGTACATTCCGATGCATGGCAAACAGATCCGTGGACTGGCGTTTAGCAGTTACCTCAGAGGCTTGCTACTCTCTGCTTCCCTAGACAACACTATTAAACTGACCAGCCTGGAGACAAATACCGTGGTCCAGACTTATAATGCTGGACGTCCTGTCTGGAGCTGTTGCTGGTGTCTTGATGAGGCTAACTACATCTATGCTGGACTGGCCAATGGTTCAATTCTGGTATATGACGTGCGAAACACGAGCAGTCATGTGCAGGAGTTAGTAGCTCAGAAAGCCAGATGCCCACTGGTCTCCCTGTCATACATGCCCAGAGCTGCCTCAGCTGCATTTCCATATGGTGGGGTGCTGGCTGGAACCTTGGAGGATGCTTCATTCTGGGAACAGAAAATGGACTTTTCTCATTGGCCTCATGTGCTGCCCTTGGAGCCAGGGGGCTGCATAGACTTTCAGACAGAGAACAGCTCCCGGCACTGTCTTGTGACCTACAGGCCTGATAAAAATCACACCACCATACGAAGTGTGCTGATGGAAATGTCCTACCGACTGGATGACACTGGAAATCCAATCTGCTCCTGCCAGCCTGTACATACATTTTTTGGAGGACCTACTTGCAAACTATTGACCAAAAATGCCATTTTCCAAAGCCCAGAGAATGATGGCAACATCCTGGTGTGTACTGGGGATGAAGCAGCAAATTCTGCCCTGCTGTGGGATGCTGCCAGTGGCTCGTTGCTCCAGGACCTACAGACCGATCAGCCTGTGTTGGACATCTGCCCATTTGAGGTGAACCGTAACAGCTACTTGGCTACCTTAACAGAGAAGATGGTCCACATCTATAAGTGGGAGTGAGCGGCCGC

Amino Acid Sequence

MMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFP
QVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVR
YNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALM
FNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDL
IKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLP
TFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP
LGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVI
NAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQG
PLGSMAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSS
QGVPSILQPAPAEVISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINP
RTSEQHRQGSDGNHTIPASSLHSMTNFISGLQRLHGMLEFLRPSSSNHSV
GPMRTRRRVSASRRARAGGSQRTDSARLRAPLDAYFQVSRTQPDLPATTY
DSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILEEQLAGV
SAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTDCLEQWTN
AGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYART
LRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRR
VQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQ
AGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMH
GKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCC
WCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPR
AASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENS
SRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTC
KLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPV
LDICPFEVNRNSYLATLTEKMVHIYKWE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU50236
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee