USP25

• Plasmid: pcDNA5D FRT/TO BirA R118G-USP25 C178A-HA (codon optimised seq)
• Parent Plasmid: pcDNA5D FRT/TO BirA R118G
• Expressed: BirA R118G-USP25 C178A-HA
• Genbank: NP_037528.3
• Tag: HA
• Tag: BirA
• Species: Human

Sequence of Insert

GGATCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAA
GCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATTACGGGGATTAATG
ACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAA
TTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGACCCCTCAGCAGGA
GGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCA
GTGTGGGAAGCCAAGCAGATACAAATGTGATTGATCTCACTGGAGATGAT
AAAGATGATCTTCAGAGAGCAATTGCCTTGAGTTTGGCCGAATCAAACAG
GGCATTCAGGGAGACTGGAATAACTGATGAGGAACAAGCCATTAGCAGAG
TTCTTGAAGCCAGCATAGCAGAGAATAAAGCATGTTTGAAGAGGACACCT
ACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGATAGAAAAAGACAGGA
CAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTgcTTGGTTTAGTG
CTGTTATTCAGTCATTATTTAATCTTTTGGAATTTAGAAGATTAGTTCTG
AATTACAAGCCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGA
ACATCGGAATTTGCCTTTTATGCGTGAGCTGAGGTATCTATTTGCACTTC
TTGTTGGTACCAAAAGGAAGTATGTTGATCCATCAAGAGCAGTTGAAATT
CTTAAGGATGCTTTCAAATCAAATGACTCACAGCAGCAAGATGTGAGTGA
GTTTACACACAAATTATTAGATTGGTTAGAAGATGCCTTCCAAATGAAAG
CTGAAGAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTG
TTCTATGGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAATTTGA
AAACACTGAAATGTTTGGTCAGTACCCACTTCAGGTCAATGGGTTCAAAG
ATCTGCATGAGTGCCTAGAAGCTGCAATGATTGAAGGAGAAATTGAGTCT
TTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAGCATTGGTTTACTGA
ATTACCACCTGTGTTAACATTTGAATTGTCAAGATTTGAATTTAATCAGG
CATTGGGAAGACCAGAAAAAATTCACAACAAATTAGAATTTCCCCAAGTT
TTATATTTGGACAGATACATGCACAGAAACAGAGAAATAACAAGAATTAA
GAGGGAAGAGATCAAGAGACTGAAAGATTACCTCACGGTATTACAACAAA
GGCTAGAAAGATATTTAAGCTATGGTTCCGGTCCCAAACGATTCCCCTTG
GTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCAAGTAAACCTGTTTG
CACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGTTCCA
TACCATCACAGACATTACCAAGCACAACAGAACAACAGGGAGCCCTATCT
TCAGAACTGCCAAGCACATCACCTTCATCAGTTGCTGCCATTTCATCGAG
ATCAGTAATACACAAACCATTTACTCAGTCCCGGATACCTCCAGATTTGC
CCATGCATCCGGCACCGAGGCACATAACGGAGGAAGAACTTTCTGTGCTG
GAAAGTTGTTTACATCGCTGGAGGACAGAAATAGAAAATGACACCAGAGA
TTTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAATTAATGTACT
CTGACAAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTT
CACGAAGGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTTGATCA
TCGTGAAAGCAGATGGATGAAGTACAATGATATTGCTGTGACAAAATCAT
CATGGGAAGAGCTAGTGAGGGACTCTTTTGGTGGTTATAGAAATGCCAGT
GCATACTGTTTAATGTACATAAATGATAAGGCACAGTTCCTAATACAAGA
GGAGTTTAATAAAGAAACTGGGCAGCCCCTTGTTGGTATAGAAACATTAC
CACCGGATTTGAGAGATTTTGTTGAGGAAGACAACCAACGATTTGAAAAA
GAACTAGAAGAATGGGATGCACAACTTGCCCAGAAAGCTTTGCAGGAAAA
GCTTTTAGCGTCTCAGAAATTGAGAGAGTCAGAGACTTCTGTGACAACAG
CACAAGCAGCAGGAGACCCAGAATATCTAGAGCAGCCATCAAGAAGTGAT
TTCTCAAAGCACTTGAAAGAAGAAACTATTCAAATAATTACCAAGGCATC
ACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGGCAATTA
AGTTGGAATATGCAAGGTTGGTTAAGTTGGCCCAAGAAGACACCCCACCA
GAAACCGATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAGAACCA
GGCACCAAAGAAAATTATTGAGAAAACATTACTAGAACAATTTGGAGATA
GAAATTTGAGTTTTGATGAAAGGTGTCACAACATAATGAAAGTTGCTCAA
GCCAAACTGGAAATGATAAAACCTGAAGAAGTAAACTTGGAGGAATATGA
GGAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTATCTCA
TAATTGGGCTAGAAAATTTTCAAAGAGAAAGTTATATAGATTCCTTGCTG
TTCCTCATCTGTGCTTATCAGAATAACAAAGAACTCTTGTCTAAAGGCTT
ATACAGAGGACATGATGAAGAATTGATATCACATTATAGAAGAGAATGTT
TGCTAAAATTAAATGAGCAAGCCGCAGAACTCTTCGAATCTGGAGAGGAT
CGAGAAGTAAACAATGGTTTGATTATCATGAATGAGTTTATTGTCCCATT
TTTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGATATACTAGCTG
TAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAA
CCACACCTCCAAGAAAAGCTGACAGATTTTTTGCCAAAACTGCTTGATTG
TTCTATGGAGATTAAAAGTTTCCATGAGCCACCGAAGTTACCTTCATATT
CCACGCATGAACTCTGTGAGCGATTTGCCCGAATCATGTTGTCCCTCAGT
CGAACTCCTGCTGATGGAAGAGCGGCCGC

Amino Acid Sequence

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMTVEQNVLQQSAAQKHQQTFLNQLREI
TGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGN
DRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQ
AISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNT
AWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRY
LFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDA
FQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQV
NGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRF
EFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLT
VLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSP
PSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRI
PPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTI
ELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIA
VTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVG
IETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESET
SVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVL
QSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLE
QFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRET
TMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHY
RRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEK
DILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPK
LPSYSTHELCERFARIMLSLSRTPADGRAAAYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24735
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee