PINK1 (Trichoplax adherens)

• Plasmid: pET6HIS SUMO Pink1 (trichoplax) D358A
• Parent Plasmid: pET6HIS SUMO
• Expressed: 6HIS SUMO pink1 trichoplax D358A
• Genbank: XP_002109370.1
• Tag: 6His
• Tag: SUMO
• Species: Trichoplax adhaerens

Sequence of Insert

GGAATGGCTCTGATTCTGCGTCGTATCTTTAACAACCTGTCGAAATTCCTGCCGCAATTTGAACCGGGCTTTGAACGTGGTGCGAAACAAGTTTTTACCG
AAAACATTAACAATGCAACGCTGACCACGCGCATTGTCAGCAAAGATAACCAGATCGTGGTTAAAAACTCGAACTACAGCAAACTGGAAAATGGCTCCGT
CTCACGTCTGCGTATCGTGGCACGTGGCCTGATTGGTAAATCTTACATCAGTAACAATCTGCACCTGATTAAGAAAATTGCATCGGCTACCCGTCTGGTT
TTTACGCGTCGCTTCCCGCTGAGCACCCCGATTTTTGCACTGCTGGGTTTCACGGTGGCAGGTCCGCGTTATAACGAACAGCAAATGGAACAGCAAAAAC
TGGTCGTGTCCCTGCAGTCACTGCTGAAAATCTGCCATAACAAACTGGAAAAAGATAATGCGGCCTCCGAAATCTCGTGCAGCTGTGACACCACGAATTC
ATATAAAGTCGGCCCGCGTCTGGGTTCTCGCAGTTGCTGTTCTGCGGTGTACGCCACCAAATGCAGTTGTGGCAAAGAATACGCGATGAAAATGATGTTC
AACTACGGTAACTCTAAAGATGACTTCATCCGCAAAAACTTCCTGCGTGAATATTGCCTGCTGAGTCCGCTGCCGGTGACGCGTGTTGATGAAATGGAAA
GCTCTAAAAACTCCCAGTCACGCATTCTGGTGCACCCGAACATCATCCGTCTGGATAAAGTTATCATCGACGAAATCCAATACTGTGATGACGCAATGCA
TGAATACCCGGATGCTCTGCCGCCGTCGCCGCTGAATCCGAGCGGTCTGGGTCGTCACCGTACCATGTTCCTGTTCATGAAAAAATACGACATGACGCTG
CGCGAATACGTTCATTCCAAAGGCATTATCCCGAACGATGAAGCAACCATGCTGCTGCTGCAGCTGCTGCAGGGTGTGGACTATCTGAACAAAAATTACA
TTGCTCACCGTGATCTGAAAAGCGACAATATTCTGATCGAAGAAGTTGATCCGTATCCGCTGCTGGCGATCACCGcCTTCGGCGAATGCCTGGATGACGC
AGCTCTGAAACTGGTTGTCCCGTACATTAGCAACTGTATCGATCGCGGCGGTAATAGTTCCCTGATGGCACCGGAAATTAAAAACGCTGAACCGGGTCAT
AATTCTGTTCTGGATTATCGTCGCAGTGACGTCTGGGCGATTGGCTGCCTGGCCTTTGAAATCTTCGGTCAGGAAAACCCGTTTCTGAATACCTTCGATT
CATCGAGCTATCAAGAATACGAACTGCCGGAACTGCCGTCTCGTGTCCCGCTGATTGTGCGTAAAATCGTTCGCAACCTGCTGCGTAAAGATTCTAGTAA
TCGTCTGCCGGCATCCCTGGCTATTGCAGCACTGTCATTTTGGCGTTGGGGTCCGATCAATTGGCGTGCACTGATGATGTATGAAACCGGTCTGAGTAAC
CTGAATGAAGAAGATATTCAGCGTTGGCTGTCGGAAACGGCCTTCCAGCTGAGCATTCAAATCATGCAGTGTCAAGCGCATGGTCACAAACTGACCCCGG
AACTGCAGGCCAAATACCATTTTCTGGTCTCAACGAGTGCCGCTGACATCAAACTGGCTGTGGAATCCCTGTATTGCTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMALILRRIFNNLSKFLPQFEPGFERGAKQVFTENINNATLTTRIVSKDNQIVVKNSNYSKLENGSVSRLRIVARGLIGKSYISNNLHLIKKIASAT
RLVFTRRFPLSTPIFALLGFTVAGPRYNEQQMEQQKLVVSLQSLLKICHNKLEKDNAASEISCSCDTTNSYKVGPRLGSRSCCSAVYATKCSCGKEYAMK
MMFNYGNSKDDFIRKNFLREYCLLSPLPVTRVDEMESSKNSQSRILVHPNIIRLDKVIIDEIQYCDDAMHEYPDALPPSPLNPSGLGRHRTMFLFMKKYD
MTLREYVHSKGIIPNDEATMLLLQLLQGVDYLNKNYIAHRDLKSDNILIEEVDPYPLLAITAFGECLDDAALKLVVPYISNCIDRGGNSSLMAPEIKNAE
PGHNSVLDYRRSDVWAIGCLAFEIFGQENPFLNTFDSSSYQEYELPELPSRVPLIVRKIVRNLLRKDSSNRLPASLAIAALSFWRWGPINWRALMMYETG
LSNLNEEDIQRWLSETAFQLSIQIMQCQAHGHKLTPELQAKYHFLVSTSAADIKLAVESLYC*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51015
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee