PINK1 (Pediculus Hum. Corp)

• Synonyms: *
• Plasmid: pet6HIS SUMO Pink1 (pediculus) D357A
• Parent Plasmid: pET6HIS SUMO
• Expressed: 6HIS SUMO pink1 pediculus D357A
• Genbank: *
• Tag: 6His
• Tag: SUMO
• Species: Pediculus humanus

Sequence of Insert

ATGTCGCTGCTGGCATACACGAACCTGCTGCTGCAAAATGGCCGCATCTTCCGTTACTACAAAAAAGCTAATATCAAAAAATTCATCAAGAAAATTATCAAACTGGATCTGAAATCCACCCCGTCAGAAGCCAGCGTGTCTCGTCAGACCTTTCTGTCGACGGGCCTGAACAGCGTTAAAAATGCGGTCCAGCTGCAAGCCCGTAAACTGCTGATTAACAATGTGCTGGAACGCGTTACCCCGACGCTGAATAGCGATCTGAAGAAAAAAGCGGCCAAACGTCTGTTCTATGGCGACTCGGCACCGTTTTTCGCTCTGGTCGGTGTGAGCCTGGCTAGTGGCTCCGGTCTGCTGACCAAAGATGACGAACTGGAAGGCATTTGCTGGGAAATCCGCGAAGCAGTTAGCAAGGGTAAATGGAACGATTCAGAATCGGAAAATGTCGAACAGCTGCAAGCAGCTAATCTGGATGAACTGGACCTGGGCGAACCGATTGCAAAAGGTTGTAACGCTGTGGTTTATTCTGCGAAACTGAAAAACGTGCAGAGTAATAAACTGGCGCACCAACTGGCCGTGAAAATGATGTTCAACTACGATGTTGAAAGCAATTCTACCGCAATTCTGAAAGCTATGTATCGTGAAACGGTTCCGGCGATGTCTTACTTTTTCAACCAGAACCTGTTTAACATCGAAAACATCAGTGATTTCAAAATTCGCCTGCCGCCGCATCCGAATATCGTTCGTATGTATAGCGTCTTTGCCGATCGCATTCCGGACCTGCAGTGCAACAAACAACTGTACCCGGAAGCACTGCCGCCGCGTATCAACCCGGAAGGCTCTGGTCGCAATATGAGTCTGTTCCTGGTCATGAAACGTTATGATTGTACCCTGAAAGAATACCTGCGTGACAAAACGCCGAACATGCGCAGCTCTATTCTGCTGCTGTCCCAGCTGCTGGAAGCAGTTGCTCATATGAACATTCACAATATCTCCCATCGCGATCTGAAATCAGACAATATCCTGGTCGATCTGAGCGAAGGCGACGCCTATCCGACCATTGTGATCACGGCTTTTGGCTGCTGTCTGTGCGACAAACAGAACGGTCTGGTGATTCCGTACCGTAGCGAAGATCAAGACAAAGGCGGTAACCGTGCCCTGATGGCACCGGAAATCGCGAATGCCAAACCGGGCACCTTCTCGTGGCTGAACTATAAAAAATCTGATCTGTGGGCGGTTGGTGCAATCGCTTACGAAATCTTCAACATCGATAACCCGTTCTACGACAAAACCATGAAACTGCTGAGTAAATCCTATAAAGAAGAAGATCTGCCGGAACTGCCGGACACGATCCCGTTTATTATCCGCAACCTGGTGTCTAATATGCTGTCACGTTCGACCAATAAACGCCTGGATTGTGATGTTGCCGCAACCGTGGCACAGCTGTATCTGTGGGCACCGAGTTCCTGGCTGAAAGAAAACTACACCCTGCCGAACAGTAATGAAATTATCCAATGGCTGCTGTGCCTGTCATCGAAAGTGCTGTGTGAACGTGATATTACGGCTCGCAACAAAACCAATACGATGAGCGAAAGCGTGAGTAAAGCGCAGTATAAAGGTCGTCGCTCCCTGCCGGAATACGAACTGATCGCGTCATTCCTGCGTCGCGTCCGTCTGCATCTGGTCCGTAAAGGTCTGAAATGGATTCAAGAACTGCACATCTACAACTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMSLLAYTNLLLQNGRIFRYYKKANIKKFIKKIIKLDLKSTPSEASVSRQTFLSTGLNSVKNAVQLQARKLLINNVLERVTPTLNSDLKKKAAKRLF
YGDSAPFFALVGVSLASGSGLLTKDDELEGICWEIREAVSKGKWNDSESENVEQLQAANLDELDLGEPIAKGCNAVVYSAKLKNVQSNKLAHQLAVKMMF
NYDVESNSTAILKAMYRETVPAMSYFFNQNLFNIENISDFKIRLPPHPNIVRMYSVFADRIPDLQCNKQLYPEALPPRINPEGSGRNMSLFLVMKRYDCT
LKEYLRDKTPNMRSSILLLSQLLEAVAHMNIHNISHRDLKSDNILVDLSEGDAYPTIVITAFGCCLCDKQNGLVIPYRSEDQDKGGNRALMAPEIANAKP
GTFSWLNYKKSDLWAVGAIAYEIFNIDNPFYDKTMKLLSKSYKEEDLPELPDTIPFIIRNLVSNMLSRSTNKRLDCDVAATVAQLYLWAPSSWLKENYTL
PNSNEIIQWLLCLSSKVLCERDITARNKTNTMSESVSKAQYKGRRSLPEYELIASFLRRVRLHLVRKGLKWIQELHIYN*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pet6HIS SUMO Pink1 (pediculus) D357A1.1 MBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU51010
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee