Parkin

• Synonyms: AR-JP; LPRS2; PDJ; PRKN; PARK2 , E2-F1; L-UBC; UBCH7; UbcM4
• Plasmid: pET15b 6His sumo UBE2L3-Parkin S127-V465(end)
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6His sumo UBE2L3-Parkin S127-V465(end)
• Genbank: NM_004562.2 GI:169790968 (Parkin), NM_003347.3 GI:373432685 with silent change a423g (UBE2L3)
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAAATCCGCAAATGTGGGATGAAAAACTTCCGTAACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCCTGACAACCCTCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGTACCCATTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGACGAAAAGGGGCAGGTCTGTCTGCCAGTAATTAGTGCCGAAAACTGGAAGCCAGCAACCAAAACCGACCAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCCCCAGCCTGAGCACCCGCTTCGGGCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCTGAGGAGTTTACAAAGAAATATGGGGAAAAGCGACCTGTGGACGGATCC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSMAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPP
YDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENW
KPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTK
KYGEKRPVDGSSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQ
CSTCRQATLTLTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAH
PTSDKETSVALHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFH
LYCVTRLNDRQFVHDPQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRY
QQYGAEECVLQMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGFAFC
RECKEAYHEGECSAVFEASGTTTQAYRVDERAAEQARWEAASKETIKKTT
KPCPRCHVPVEKNGGCMHMKCPQPQCRLEWCWNCGCEWNRVCMGDHWFDV
*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU50097
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee