PINK1

• Plasmid: pcDNA5 FRT/TO FLAG Pink1 110-581 Fis1 101-end
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: FLAG-PINK1 (110-581)-FIS1 (101-end)
• Genbank: Q9BXM7
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCACCATGCTCATCGAGGAAAAACAGGCGGAGAGCCGGCGGGC
GGTCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAAGC
CGGGGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGCTGGAG
GAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTGTA
TGAAGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGACAAAGAGCA
CCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGG
CAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATGAT
GTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGA
GCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGA
GCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCTCA
CCCCAACATCATCCGGGTTCTCCGCGCCTTCACCTCTTCCGTGCCGCTGC
TGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCCAC
CCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAACTA
TCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCC
TCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTT
CAACAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTGTGGA
GCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTTTGGCTGCT
GCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCTGGTAC
GTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACGGC
CCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTGGG
CAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCTAC
GGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCTCAGCT
ACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGTGAGGG
CACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAGCCGCA
AATGTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAGAA
TCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGCCA
CTTTGTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAACAAAAATG
AAGATGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGCCAGGCAGC
CCTCCTCCTCTGCTCATGGAGGGCAGCCCTGGCTAGCGAGCCCCAGAACA
ACCAGGCCAAGGAACTGGAGCGGCTCATTGACAAGGCCATGAAGAAAGAT
GGACTCGTGGGCATGGCCATCGTGGGAGGCATGGCCCTGGGTGTGGCGGG
ACTGGCCGGACTCATCGGACTTGCTGTGTCCAAGTCCAAATCCTGAGCGG
CCGC

Amino Acid Sequence

MLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLI
GQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERA
PGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTY
RKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGL
GHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGI
AHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRG
GNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGK
AHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLH
LSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLF
LANLECETLCQAALLLCSWRAALASEPQNNQAKELERLIDKAMKKDGLVG
MAIVGGMALGVAGLAGLIGLAVSKSKS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46897
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee