Pink1 (Nasonia)

• Plasmid: pET156P SUMO Pink1 (Nasonia) codon opt
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO Pink1 (Nasonia) codon opt
• Tag: 6His
• Tag: SUMO
• Species: Nasonia vitripennis

Sequence of Insert

GGATCCATGAGCATTCGTACCGCAGCACAGCGTCTGATTCAGGATGGTCGTCTGCTGCTGCGTAGCCTGCGTAATCCGGAACATTATCGTAATACCACCC
GTGGCCATTTTAACAAAATTCATGTTGCACGTGTTGGTGAAAGCCAGGGTCATGTTCCGAAAGGCACCGGTGATGTTAGCAGCGCACGTAGCCATCTGGG
TTTTCTGGGTGCACAGGCACGTCGTCTGTTTGTTGATAGCATTCTGAAACGTGTTACCAATACCCTGGCAGCCGATCTGCGTCGTCGTGCAGCAAGCCGT
CTGCTGTTTGGTGGTGATAGCGCACCGTTTTTTGCACTGGTTGGTATTAGCCTGGCAAGCGGCACCGGTATTCTGACCAAAGAAGATGAACTGGAAGGCG
TTTGTTGGGAAATTCGTGAAGCAGTTATGAAACTGCAGTGGAATAAACCGCGTAACGACAAAAACTATGAAACCACCCTGACCGATGATAATCAGATTAG
CCTGCAGAATTTTGTGATTGGTCCGCTGATTGCAAAAGGTTGTAGCGCAGTTGTTTATGCAGCACGTACCAAAAATAGTGATGATAACCAGAACAGCGAA
GAGATTACCATTGATCGTAGCAGCAATATTACCAGCTTTCCGCTGGCAATCAAAATGATGTTTAACTATGATGCCGAAAGCAATGCAACCGCAATTCTGC
GTGCAATGTTTCGTGAAACCGTTCCGGCATGGAAACACTATAAAAACGAAGAACTGAGCCACTGGGAACAGAAAATGGCAGATAACAAAAAAACCCTGCC
TGCCCATAGCAATATTGTTGCAATGTATAGCGTTTTTGCAGATCGTGTTCCGTTTCTGCCTGGTAGCATTAAAATGTATCCGGATGCACTGCCTGCACGT
CTGAATCCGGAAGGTAGCGGTCGTAATATGAGCCTGTTTCTGCTGATGAAACGTTATGATGTTACCCTGAAACAGTATGTTAGCGATCGTCAGCTGAATA
CCCGTGAAAGCATTCTGCTGTTCGCACAGCTGCTGGAAGGTGTTGCACATATGAATAGCCATGGTATTGCACATCGTGATCTGAAAGCAGATAATATCCT
GCTGGATATCAGCGAAGAAAGCGATAATTGTCCGACCCTGGTTATTACCGATTTTGGTTGTTGTCTGGCCGATAAAAATCATGGTCTGTATCTGCCGTAT
AACACCCATGATACCGATAAAGGTGGTAATGCAGCACTGATGGCACCGGAAATTGTTACCGCAGAACCGGGTCCGTTTACCAGCCTGAATTATACCAAAA
GCGATCTGTGGACCGTTGGCACCATTGCATATGAAATTTTTGGCATGAAAAACCCGTTTTATGGCAGCGTTAAAGAAAAAGCCATGCTGAAAAACTACGA
TTATAAAGTCGAAGATCTGCCTCCGCTGCCGGAAACCGTGCCGGAAATCATTAGCGCACTGGTTCGTAATATTCTGACACGTAGCACCTATAAACGTCTG
AGCGTTGAAATGGCAGCAACCATTATGCAGCTGTTTCTGTGGGCACCGAGCAGCTGGCTGCAGAAAGATAGCAAAATTCCGAGCAGCAGCGAAATCCTGC
AGTGGCTGCTGTGTCTGACCACCAAAGTTCTGTGTGAAGGTCGTAATGATAGTCTGGAAGAACGCAATAGCATTGATGCACAGAAAACCGAAAGTCCGCA
TCAGCTGAGCTATCGTCGTAGCCTGAGCACCCGTAGCTGTGGTCGTCGTACAATGCCGGAATATCAACTGATTGCGAGCTTTCTGGGTCGTGTTAGCCTG
TTTAATATCCGCGAAGGTTTTAAATGGATTCAGCGCAACAACTACGTGTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMSIRTAAQRLIQDGRLLLRSLRNPEHYRNTTRGHFNKIHVARVGESQGHVPKGTGDVSSARSHLGFLGAQAR
RLFVDSILKRVTNTLAADLRRRAASRLLFGGDSAPFFALVGISLASGTGILTKEDELEGVCWEIREAVMKLQWNKPRNDKNYETTLTDDNQISLQNFVIG
PLIAKGCSAVVYAARTKNSDDNQNSEEITIDRSSNITSFPLAIKMMFNYDAESNATAILRAMFRETVPAWKHYKNEELSHWEQKMADNKKTLPAHSNIVA
MYSVFADRVPFLPGSIKMYPDALPARLNPEGSGRNMSLFLLMKRYDVTLKQYVSDRQLNTRESILLFAQLLEGVAHMNSHGIAHRDLKADNILLDISEES
DNCPTLVITDFGCCLADKNHGLYLPYNTHDTDKGGNAALMAPEIVTAEPGPFTSLNYTKSDLWTVGTIAYEIFGMKNPFYGSVKEKAMLKNYDYKVEDLP
PLPETVPEIISALVRNILTRSTYKRLSVEMAATIMQLFLWAPSSWLQKDSKIPSSSEILQWLLCLTTKVLCEGRNDSLEERNSIDAQKTESPHQLSYRRS
LSTRSCGRRTMPEYQLIASFLGRVSLFNIREGFKWIQRNNYV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46787
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee