Parkin (tribolium)

• Plasmid: pET156P SUMO Parkin (tribolium)
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO parkin (tribolium)
• Genbank: XP_969040.2
• Tag: 6His
• Tag: SUMO
• Species: Tribolium castaneum

Sequence of Insert

GGATCCATGATGAGCTTTATCATCAACGTGATTAAAGATCTGCTGCATAAAATGCTGCAGTTTCTGAGCTTTAGCCGTAAAACCCTGAGCAATAATCTGA
GCATCTACATCAAAACCAATACCGGTAATACCCTGAGTGTTGATCTGGACCCGACCTGGGATATTAAAGAAGTGAAAGAATTTGTTGCACCGCAGCTGGG
TCTGAAACCGGGTGAAGTTAAAATCATTTTTGCCGGTAAAGAACTGGGCGATAACATTAGCATTAGCGAATGTGATCTGGGTCAGCAGAGCACCCTGCAT
GCAATCAAAATTACCAAAAAACCGAATCCGCCTACCCTGAATAATGCCGTTATTGAAGAAAGTGATGAAGGTAAACCGCTGTGTGAAACACTGAGCGATC
TGAGTTTTAGCAATGATGATAGCGAAGAAGGTCCGACCGAACAGCGTAAAGTTCATTTTTTTGTTTATTGCCCGACCTGCAAAGCCCTGAAAAATGGTAA
ACTGCGTGTTCGTTGCCACTTTTGTAAAAGCGGTGCATTTACCGTTCATAGCGATCCGCAGAATTGGAATGATGTTCTGCAGAGCAAACAAATTACCGGT
CTGTGCGAAAATAATCCGGAACTGTGTGCAAATGTGCTGAATAACAAAGAACCGACCTTTGCCGAGTTCTATTTCAAATGTAGCGAACATACCAGCCTGG
GTGAAACCGATGAAGCAGTTCCGCTGTATCTGATTCGTCCGAATCTGAAAGAGGTTCCTTGTCTGGCATGTGCCGATATTAATGAAACCGTTTTTGTGTT
TCCGTGCAAAGAAGGTCACGTTACCTGTCTGGATTGTTTTCGTCAGTATTGTACCACCCGTCTGCGTGAACGTCAGTTTTGGCAGCATCCGGAATTTGGT
TATACCCTGGCATGTCCGGCAGGTTGTCCGGATAGCTTTATTCAAGAAATTCATCATTTTCGCCTGCTGAGCGAAAGCCAGTATACCCAGTATCAGCGTT
TTGCAACCGAAGAATATGTGCTGCGTAGCGGTGGTGTTCTGTGTCCGCAGCCTGGTTGTGGTATGGGTATTCTGGCCGATCCGGAATGTACCAAAATCAC
CTGTATTAATGGTTGCGGCTATGTGTTTTGTCGTCTGTGTCTGCAGGGTTTTCATATTGGTGAATGTGAAGCACCGGAAGGTGATGTTGGTGCACCGGGT
GATTGTAGCTATAATGTTGATCCGACCCGTGCAGCCGAAGCACGTTGGGATGAAGCAAGCAAAGTTGCAATTAAAGTTCTGACCAAACCGTGTCCGAAAT
GTCGTACCCCGACAGAACGTGATGGTTAAGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMMSFIINVIKDLLHKMLQFLSFSRKTLSNNLSIYIKTNTGNTLSVDLDPTWDIKEVKEFVAPQLGLKPGEVKIIFAGKELGDNISISECDLGQQST
LHAIKITKKPNPPTLNNAVIEESDEGKPLCETLSDLSFSNDDSEEGPTEQRKVHFFVYCPTCKALKNGKLRVRCHFCKSGAFTVHSDPQNWNDVLQSKQI
TGLCENNPELCANVLNNKEPTFAEFYFKCSEHTSLGETDEAVPLYLIRPNLKEVPCLACADINETVFVFPCKEGHVTCLDCFRQYCTTRLRERQFWQHPE
FGYTLACPAGCPDSFIQEIHHFRLLSESQYTQYQRFATEEYVLRSGGVLCPQPGCGMGILADPECTKITCINGCGYVFCRLCLQGFHIGECEAPEGDVGA
PGDCSYNVDPTRAAEARWDEASKVAIKVLTKPCPKCRTPTERDG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46750
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee