PINK1

• Plasmid: pcDNA5 FRTTO Pink1 3FLAG Tribolium E123K E124K E125K E127K
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: Pink1 3FLAG Tribolium E123K E124K E125K E127K
• Genbank: XP_968367 (codon-optimised)
• Tag: 3FLAG
• Species: Tribolium castaneum

Sequence of Insert

GGATCCATGAGCGTCCGTGCAGTTGGCAGCCGCCTGTTCAAACATGGCCGTAGCCTGATCCAGCAGTTCTGTAAACGTGACCTGAATACCACCATTGGCG
ATAAAATCAATGCCGTGAGCCAGGCAACCGCCGCACCGAGCTCTCTGCCGAAAACCCAAATTCCGAAAAACTTTGCCCTGCGTAATGTCGGCGTGCAGCT
GGGTCTGCAAGCACGTCGCATTCTGATCGATAACGTCCTGAATCGTGTGACCAATTCACTGTCGGCCGAACTGCGCAAAAAAGCAACGCGTCGCATCCTG
TTCGGCGACAGCGCACCGTTTTTCGCTCTGGTTGGTGTCAGTATTGCCTCCGGCACCGGTATCCTGACGAAAaAAaAAaAACTGaAAGGCGTTTGCTGGG
AAATCCGTGAAGCGATCTCTAAAATCAAATGGCAGTACTACGATATCGACGAAAGCCGCTTTGAATCTAACCCGATCACCCTGAATGATCTGAGTCTGGG
TAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATG
TTCAACTACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACA
ATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGA
AGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGAT
TGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACG
GTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGATTTTGGCTGCTGTCTGGC
GGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCG
GGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGT
CTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACG
TAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCG
ACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTA
ATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAA
CCTGCCGGAACTGGATGCGGCCGC

Amino Acid Sequence

MSVRAVGSRLFKHGRSLIQQFCKRDLNTTIGDKINAVSQATAAPSSLPKTQIPKNFALRNVGVQLGLQARRILIDNVLNRVTNSLSAELRKKATRRILFG
DSAPFFALVGVSIASGTGILTKKKKLKGVCWEIREAISKIKWQYYDIDESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKMMFN
YDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKRYDCN
LQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDTSEPESPILVISDFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQKPGT
FSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLKVPTS
GEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISSFLCRAKLANVRNALHWIQENLPELDGGRDYKDDDDKDYKDDDDKDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46852
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee