RFWD3

• Synonyms: RNF201
• Plasmid: pFastbac HTb RFWD3
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-RFWD3
• Genbank: NM_018124.3 GI:71143111
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCTCATGAAGCAATGGAATATGATGTTCAGGTGCAGTTAAATCATGCCGAACAACAGCCAGCTCCTGCTGGCATGGCCAGCAGCCAAGGGGGACCAGCCCTCCTCCAGCCTGTTCCTGCTGATGTGGTCAGCAGCCAGGGGGTACCATCCATCCTCCAGCCAGCTCCTGCTGAGGTGATCAGCAGCCAAGCGACACCACCCCTGCTCCAGCCTGCTCCGCAACTGTCTGTTGACCTGACAGAAGTGGAGGTCTTGGGAGAAGACACTGTGGAGAACATCAATCCAAGAACTTCAGAACAACATAGGCAGGGATCTGATGGTAATCACACCATCCCAGCATCTTCGTTGCATTCAATGACCAACTTCATCAGCGGACTGCAGAGACTTCATGGCATGCTGGAATTCCTGAGACCTTCATCTTCAAACCACAGTGTAGGGCCAATGAGAACAAGAAGGAGGGTATCTGCTTCACGGAGGGCAAGAGCCGGAGGGTCTCAGAGGACAGACAGTGCCAGGTTGAGAGCACCATTGGATGCTTACTTTCAGGTGAGCAGGACCCAGCCTGACTTGCCAGCTACCACTTATGATTCAGAGACTAGGAATCCTGTATCTGAAGAGTTGCAGGTGTCTAGTAGTTCTGATTCTGACAGTGACAGCTCTGCAGAGTATGGAGGGGTTGTTGACCAGGCAGAGGAATCTGGAGCTGTCATTTTAGAAGAGCAACTAGCAGGTGTCTCAGCAGAGCAAGAAGTTACATGTATCGATGGAGGCAAGACCCTCCCCAAACAGCCATCTCCCCAGAAGTCTGAGCCTCTGCTACCTTCTGCTTCTATGGATGAGGAAGAAGGGGACACTTGTACAATATGTCTGGAACAGTGGACCAATGCTGGGGACCACCGGCTCTCAGCATTACGCTGTGGGCATCTCTTTGGGTATAGGTGCATTTCCACGTGGCTTAAAGGACAAGTACGAAAATGTCCCCAGTGCAACAAGAAAGCCAGGCACAGTGACATTGTCGTCCTTTATGCCCGAACCCTGAGAGCTTTGGACACTAGTGAACAGGAGCGCATGAAAAGTTCCCTACTGAAGGAACAGATGCTAAGGAAACAGGCCGAGTTAGAATCAGCACAGTGCCGACTCCAACTGCAGGTCCTCACTGATAAGTGCACTAGGCTTCAAAGGCGTGTTCAGGACTTGCAAAAACTTACGTCACATCAAAGTCAGAATTTACAGCAACCCAGGGGCTCCCAAGCATGGGTCCTGAGCTGCTCACCCTCCAGCCAGGGCCAGCACAAGCACAAGTACCACTTCCAAAAGACCTTCACAGTATCTCAGGCAGGAAACTGCCGGATCATGGCATACTGTGATGCTCTGAGCTGCCTGGTGATATCACAGCCTTCTCCTCAGGCCTCTTTTCTTCCAGGCTTTGGTGTTAAGATGTTGAGTACTGCCAACATGAAGAGCAGTCAGTACATTCCGATGCATGGCAAACAGATCCGTGGACTGGCGTTTAGCAGTTACCTCAGAGGCTTGCTACTCTCTGCTTCCCTAGACAACACTATTAAACTGACCAGCCTGGAGACAAATACCGTGGTCCAGACTTATAATGCTGGACGTCCTGTCTGGAGCTGTTGCTGGTGTCTTGATGAGGCTAACTACATCTATGCTGGACTGGCCAATGGTTCAATTCTGGTATATGACGTGCGAAACACGAGCAGTCATGTGCAGGAGTTAGTAGCTCAGAAAGCCAGATGCCCACTGGTCTCCCTGTCATACATGCCCAGAGCTGCCTCAGCTGCATTTCCATATGGTGGGGTGCTGGCTGGAACCTTGGAGGATGCTTCATTCTGGGAACAGAAAATGGACTTTTCTCATTGGCCTCATGTGCTGCCCTTGGAGCCAGGGGGCTGCATAGACTTTCAGACAGAGAACAGCTCCCGGCACTGTCTTGTGACCTACAGGCCTGATAAAAATCACACCACCATACGAAGTGTGCTGATGGAAATGTCCTACCGACTGGATGACACTGGAAATCCAATCTGCTCCTGCCAGCCTGTACATACATTTTTTGGAGGACCTACTTGCAAACTATTGACCAAAAATGCCATTTTCCAAAGCCCAGAGAATGATGGCAACATCCTGGTGTGTACTGGGGATGAAGCAGCAAATTCTGCCCTGCTGTGGGATGCTGCCAGTGGCTCGTTGCTCCAGGACCTACAGACCGATCAGCCTGTGTTGGACATCTGCCCATTTGAGGTGAACCGTAACAGCTACTTGGCTACCTTAACAGAGAAGATGGTCCACATCTATAAGTGGGAGTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMAHEAMEYDVQVQLNHAEQQPA
PAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEVISSQATPPLLQP
APQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSMTN
FISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSA
RLRAPLDAYFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSA
EYGGVVDQAEESGAVILEEQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEP
LLPSASMDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKG
QVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQA
ELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVL
SCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQAS
FLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTI
KLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNT
SSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKM
DFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSY
RLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEA
ANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYK
WE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU49558
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee