PINK1 (Drosophila)

• Plasmid: pET15b 6His SUMO Pink1 drosophila 182-721
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO Pink1 drosophila 182-721
• Genbank: Q0KHV6
• Tag: 6His
• Tag: SUMO
• Species: Drosophila Melanogaster

Sequence of Insert

GGATCCGATACGCTAGACAGCAAGTTCACCATCGATGACTTGGAAAT
CGGTCCGCCCATAGCCAAAGGTTGTGCCGCTGTCGTCTATGCAGCGGATT
TCAAGAAAGATGTTGCCTCGGATGGTGCATCCCTGCATACCGATGCGCAG
CCGCAGGCAACGCCAGCCTTTGCGCCGAATAGCTGGAGTACCCACGAGAT
GATGTCGCCGCTGCAGAACATGTCGCGCTTTGTTCACAACTTTGGCGGCT
CTGTGGACAACGTCTTCCACTACAGCCAGCCATCTGCGGCCAGTGATTTC
GTGGGCGCCCAGTCGAGGGAACAGGACCAGCGGCACCACGAGCAACAGCA
GCATCAGAATCAGGAACAAGAGCAGCATCAGAACCAGGAGCCCAGCAGCA
GTGCCTTCAATGTGACTTCTCCAGCGAATTCAAACATCAACAGCTCTGTG
GACAGTTATCCACTGGCACTCAAAATGATGTTCAACTACGACATCCAGAG
CAACGCTCTGTCCATACTGCGTGCCATGTACAAGGAGACGGTACCGGCAC
GCCAGCGCGGCATGAATGAGGCCGCCGATGAGTGGGAACGTTTGCTGCAG
AATCAAACTGTTCACCTGCCGCGGCATCCGAACATTGTCTGTATGTTTGG
CTTCTTTTGCGACGAGGTGCGCAACTTTCCCGATGGCCATCTCCTGTATC
CGGTGGCGCAGCCGCAACGCATCAATCCACAGGGCTATGGCCGCAATATG
TCGCTGTATCTGCTGATGAAACGCTACGATCACAGTCTTCGCGGCCTGCT
CGATAGCCAGGATCTGAGCACGCGCAATCGCATCCTGCTGCTGGCTCAAA
TGCTCGAGGCTGTCAACCATTTGAGCCGTCACGGCGTTGCCCATCGTGAC
CTAAAGTCTGATAATGTGCTAATCGAGCTGCAGGACGATGCGGCGCCGGT
GCTAGTGCTCTCCGACTTTGGCTGTTGTCTGGCGGACAAGGTGCATGGCC
TGCGCCTGCCGTACGTTTCGCACGACGTCGACAAGGGCGGCAATGCGGCG
TTGATGGCGCCAGAGATCTTCAATACGATGCCCGGTCCGTTTGCCGTACT
CAATTATGGCAAGGCCGATCTGTGGGCTTGCGGTGCCCTGGCTTACGAAA
TCTTTGGCAACCGCAATCCGTTCTATTCGAGCAGCGGTGGGATGGCGCGC
GAACGCGGTGAGATGACGCTTTCGCTGAGGAACAGCGATTACCGACAGGA
CCAATTGCCGCCAATGAGCGATGCTTGCCCGCCGCTGCTGCAACAGCTGG
TCTACAACATCCTCAATCCCAACCCGTCCAAGCGGGTCAGTCCGGATATT
GCGGCAAATGTGGTGCAGCTGTTCCTCTGGGCCCCATCCAATTGGCTTAA
GGCGGGCGGCATGCCCAACAGTCCGGAGATCCTACAGTGGCTCCTTTCGC
TGACCACTAAGATTATGTGCGAGGGTCGTCCACAGATGGGCGCCGGATTG
ATGCCAGTGGCCAGCTGTGGCAATAGGCGCGCCTATGTGGAGTACCTGCT
CATATGCAGCTTTCTGGCACGCGCCCGGCTGCGTCGCATTCGCGGCGCCC
TCAACTGGATACAGAATGTGGTGGCGTAGGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGDTLDSKFTIDDLEIGPPIAKGCAAVVYAADFKKDVASDGASLHTDA
QPQATPAFAPNSWSTHEMMSPLQNMSRFVHNFGGSVDNVFHYSQPSAASD
FVGAQSREQDQRHHEQQQHQNQEQEQHQNQEPSSSAFNVTSPANSNINSS
VDSYPLALKMMFNYDIQSNALSILRAMYKETVPARQRGMNEAADEWERLL
QNQTVHLPRHPNIVCMFGFFCDEVRNFPDGHLLYPVAQPQRINPQGYGRN
MSLYLLMKRYDHSLRGLLDSQDLSTRNRILLLAQMLEAVNHLSRHGVAHR
DLKSDNVLIELQDDAAPVLVLSDFGCCLADKVHGLRLPYVSHDVDKGGNA
ALMAPEIFNTMPGPFAVLNYGKADLWACGALAYEIFGNRNPFYSSSGGMA
RERGEMTLSLRNSDYRQDQLPPMSDACPPLLQQLVYNILNPNPSKRVSPD
IAANVVQLFLWAPSNWLKAGGMPNSPEILQWLLSLTTKIMCEGRPQMGAG
LMPVASCGNRRAYVEYLLICSFLARARLRRIRGALNWIQNVVA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46332
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee