PINK1 (Pediculus Hum. Corp)

• Synonyms: PARK6
• Plasmid: pET6HIS SUMO pink1 pediculus 148-575 D357A
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO pink1 pediculus 148-575 D357A
• Genbank: XP_002432225
• Tag: 6His
• Tag: SUMO
• Species: Pediculus humanus

Sequence of Insert

GTCGAACAGCTGCAAGCAGCTAATCTGGATGAACTGGACCTGGGCGAACC
GATTGCAAAAGGTTGTAACGCTGTGGTTTATTCTGCGAAACTGAAAAACG
TGCAGAGTAATAAACTGGCGCACCAACTGGCCGTGAAAATGATGTTCAAC
TACGATGTTGAAAGCAATTCTACCGCAATTCTGAAAGCTATGTATCGTGA
AACGGTTCCGGCGATGTCTTACTTTTTCAACCAGAACCTGTTTAACATCG
AAAACATCAGTGATTTCAAAATTCGCCTGCCGCCGCATCCGAATATCGTT
CGTATGTATAGCGTCTTTGCCGATCGCATTCCGGACCTGCAGTGCAACAA
ACAACTGTACCCGGAAGCACTGCCGCCGCGTATCAACCCGGAAGGCTCTG
GTCGCAATATGAGTCTGTTCCTGGTCATGAAACGTTATGATTGTACCCTG
AAAGAATACCTGCGTGACAAAACGCCGAACATGCGCAGCTCTATTCTGCT
GCTGTCCCAGCTGCTGGAAGCAGTTGCTCATATGAACATTCACAATATCT
CCCATCGCGATCTGAAATCAGACAATATCCTGGTCGATCTGAGCGAAGGC
GACGCCTATCCGACCATTGTGATCACGGCTTTTGGCTGCTGTCTGTGCGA
CAAACAGAACGGTCTGGTGATTCCGTACCGTAGCGAAGATCAAGACAAAG
GCGGTAACCGTGCCCTGATGGCACCGGAAATCGCGAATGCCAAACCGGGC
ACCTTCTCGTGGCTGAACTATAAAAAATCTGATCTGTGGGCGGTTGGTGC
AATCGCTTACGAAATCTTCAACATCGATAACCCGTTCTACGACAAAACCA
TGAAACTGCTGAGTAAATCCTATAAAGAAGAAGATCTGCCGGAACTGCCG
GACACGATCCCGTTTATTATCCGCAACCTGGTGTCTAATATGCTGTCACG
TTCGACCAATAAACGCCTGGATTGTGATGTTGCCGCAACCGTGGCACAGC
TGTATCTGTGGGCACCGAGTTCCTGGCTGAAAGAAAACTACACCCTGCCG
AACAGTAATGAAATTATCCAATGGCTGCTGTGCCTGTCATCGAAAGTGCT
GTGTGAACGTGATATTACGGCTCGCAACAAAACCAATACGATGAGCGAAA
GCGTGAGTAAAGCGCAGTATAAAGGTCGTCGCTCCCTGCCGGAATACGAA
CTGATCGCGTCATTCCTGCGTCGCGTCCGTCTGCATCTGGTCCGTAAAGG
TCTGAAATGGATTCAAGAACTGCACATCTACAACTAA

Amino Acid Sequence

MGHHHHHYSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGVEQLQAANLDELDLGEPIAKGCNAVVYSAKLKNVQSNKLAHQLAVK
MMFNYDVESNSTAILKAMYRETVPAMSYFFNQNLFNIENISDFKIRLPPH
PNIVRMYSVFADRIPDLQCNKQLYPEALPPRINPEGSGRNMSLFLVMKRY
DCTLKEYLRDKTPNMRSSILLLSQLLEAVAHMNIHNISHRDLKSDNILVD
LSEGDAYPTIVITAFGCCLCDKQNGLVIPYRSEDQDKGGNRALMAPEIAN
AKPGTFSWLNYKKSDLWAVGAIAYEIFNIDNPFYDKTMKLLSKSYKEEDL
PELPDTIPFIIRNLVSNMLSRSTNKRLDCDVAATVAQLYLWAPSSWLKEN
YTLPNSNEIIQWLLCLSSKVLCERDITARNKTNTMSESVSKAQYKGRRSL
PEYELIASFLRRVRLHLVRKGLKWIQELHIYN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46989
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee