PINK1 (C.elegans)

• Plasmid: pET6HIS SUMO Pink1 C elegans 129-641 D361A
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO Pink1 c elegans129-641 D361A
• Genbank: NP_495017
• Tag: 6His
• Tag: SUMO
• Species: Caenorhabditis elegans

Sequence of Insert

ggaGAATGGCCGAATAGAATTGATTCTTATGAGTTTGGGGAATTTCTCGG
TCAAGGATGCAATGCAGCAGTTTACTCTGCGAGATTAGCCAATTCTGATG
CAGAATCCTCAGGGAATACTCACTATGGTGCAGGGTTTAATGAAGTCACA
AATATACTTGCAGAAATTCCGCCAGTTAGCAAAGTTGCACAAAAGAAATT
CCCGTTGGCAATCAAATTAATGTTTAATTTTGAACATGATCGCGATGGAG
ATGCTCATCTCTTGAAATCAATGGGAAATGAATTGGCTCCATATCCGAAT
GCTGCAAAGTTGCTCAATGGACAAATGGGAACATTTAGACCTCTTCCAGC
AAAACATCCAAATGTTGTTCGAATTCAGACAGCTTTTATTGATTCGTTAA
AAGTTTTGCCAGATGCGATTGAACGATATCCAGATGCCCTTCACACTGCA
CGTTGGTATGAGTCAATTGCCTCCGAACCGAAAACAATGTACGTAGTAAT
GAGACGATACCGACAAACACTTCATGAATATGTATGGACTCGTCATCGAA
ATTATTGGACAGGACGAGTGATAATTGCTCAACTATTAGAAGCATGTACA
TATCTTCATAAGCATAAAGTTGCTCAGCGAGACATGAAAAGTGATAATAT
TCTTCTGGAATATGATTTTGACGACGAGATTCCCCAATTAGTTGTCGCCG
CTTTTGGATGTGCACTTGCATGTGACAATTGGCAAGTAGACTATGAATCA
GATGAAGTTAGTCTTGGAGGAAATGCCAAGACAAAAGCACCAGAAATTGC
GACGGCTGTTCCTGGAAAGAATGTAAAAGTAAACTTCGAAATGGCAGATA
CATGGGCAGCTGGAGGCCTTTCTTATGAAGTTCTAACACGATCAAATCCA
TTCTACAAACTTCTTGATACTGCAACATACCAGGAATCAGAACTACCAGC
ACTCCCATCTCGTGTCAATTTTGTGGCACGAGATGTCATTTTTGACCTAC
TCAAGCGAGATCCTAATGAAAGAGTCAAGCCGAATATTGCTGCAAATGCG
TTGAATTTGTCATTGTTCAGAATGGGAGAAGATGTGAAGCAGATGATGGA
AAAATGTGGAATATCTCAAATGACTACTCTATTGGCTGGAAGTTCTAAAG
TTTTGAGTCAAAAAATCAATAGTCGTCTGGACAAAGTGATGAATCTGATT
ACTGCTGAAACTATCATGGCCAACCTAGCTCCACATTTGATTAGTCGAGC
AGAACGACAACTTCGAGCAACATTTCTTTCAAGAATGAATCGAGAAGATA
TTTGGAGAAGTCTTCAATATTTCTTCCCAGCTGGTGTTCAACTTGACACA
CCTGCCACATCATCAGACTGTTTGGAGACTATTTCCAGTTTGATGTCGAG
TTTTTCAAATGATTCAGAAAATTACGAGAAGCAACAGAAACCGGCTAAAA
ATGGATACAACAATGTTCCACTTCTTCTCAGAAATGTTATCCGTACAGAT
GCGGATGGAATCAATGGAATTGTACATAGAGTTCGATCTAAATAGgcggc
cgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHSPKELGMEEEDVIEVYQE
QTGGEWPNRIDSYEFGEFLGQGCNAAVYSARLANSDAESSGNTHYGAGFN
EVTNILAEIPPVSKVAQKKFPLAIKLMFNFEHDRDGDAHLLKSMGNELAP
YPNAAKLLNGQMGTFRPLPAKHPNVVRIQTAFIDSLKVLPDAIERYPDAL
HTARWYESIASEPKTMYVVMRRYRQTLHEYVWTRHRNYWTGRVIIAQLLE
ACTYLHKHKVAQRDMKSDNILLEYDFDDEIPQLVVAAFGCALACDNWQVD
YESDEVSLGGNAKTKAPEIATAVPGKNVKVNFEMADTWAAGGLSYEVLTR
SNPFYKLLDTATYQESELPALPSRVNFVARDVIFDLLKRDPNERVKPNIA
ANALNLSLFRMGEDVKQMMEKCGISQMTTLLAGSSKVLSQKINSRLDKVM
NLITAETIMANLAPHLISRAERQLRATFLSRMNREDIWRSLQYFFPAGVQ
LDTPATSSDCLETISSLMSSFSNDSENYEKQQKPAKNGYNNVPLLLRNVI
RTDADGINGIVHRVRSK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46194
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee