PINK1 (C.elegans)

• Plasmid: pET6HIS SUMO Pink1 C elegans 129-641
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO Pink1 c elegans129-641
• Genbank: NP_495017
• Tag: 6His
• Tag: SUMO
• Species: Caenorhabditis elegans

Sequence of Insert

ggaGAATGGCCGAATAGAATTGATTCTTATGAGTTTGGGGAATTTCT CGGTCAAGGATGCAATGCAGCAGTTTACTCTGCGAGATTAGCCAATTCTG ATGCAGAATCCTCAGGGAATACTCACTATGGTGCAGGGTTTAATGAAGTC ACAAATATACTTGCAGAAATTCCGCCAGTTAGCAAAGTTGCACAAAAGAA ATTCCCGTTGGCAATCAAATTAATGTTTAATTTTGAACATGATCGCGATG GAGATGCTCATCTCTTGAAATCAATGGGAAATGAATTGGCTCCATATCCG AATGCTGCAAAGTTGCTCAATGGACAAATGGGAACATTTAGACCTCTTCC AGCAAAACATCCAAATGTTGTTCGAATTCAGACAGCTTTTATTGATTCGT TAAAAGTTTTGCCAGATGCGATTGAACGATATCCAGATGCCCTTCACACT GCACGTTGGTATGAGTCAATTGCCTCCGAACCGAAAACAATGTACGTAGT AATGAGACGATACCGACAAACACTTCATGAATATGTATGGACTCGTCATC GAAATTATTGGACAGGACGAGTGATAATTGCTCAACTATTAGAAGCATGT ACATATCTTCATAAGCATAAAGTTGCTCAGCGAGACATGAAAAGTGATAA TATTCTTCTGGAATATGATTTTGACGACGAGATTCCCCAATTAGTTGTCG CCGATTTTGGATGTGCACTTGCATGTGACAATTGGCAAGTAGACTATGAA TCAGATGAAGTTAGTCTTGGAGGAAATGCCAAGACAAAAGCACCAGAAAT TGCGACGGCTGTTCCTGGAAAGAATGTAAAAGTAAACTTCGAAATGGCAG ATACATGGGCAGCTGGAGGCCTTTCTTATGAAGTTCTAACACGATCAAAT CCATTCTACAAACTTCTTGATACTGCAACATACCAGGAATCAGAACTACC AGCACTCCCATCTCGTGTCAATTTTGTGGCACGAGATGTCATTTTTGACC TACTCAAGCGAGATCCTAATGAAAGAGTCAAGCCGAATATTGCTGCAAAT GCGTTGAATTTGTCATTGTTCAGAATGGGAGAAGATGTGAAGCAGATGAT GGAAAAATGTGGAATATCTCAAATGACTACTCTATTGGCTGGAAGTTCTA AAGTTTTGAGTCAAAAAATCAATAGTCGTCTGGACAAAGTGATGAATCTG ATTACTGCTGAAACTATCATGGCCAACCTAGCTCCACATTTGATTAGTCG AGCAGAACGACAACTTCGAGCAACATTTCTTTCAAGAATGAATCGAGAAG ATATTTGGAGAAGTCTTCAATATTTCTTCCCAGCTGGTGTTCAACTTGAC ACACCTGCCACATCATCAGACTGTTTGGAGACTATTTCCAGTTTGATGTC GAGTTTTTCAAATGATTCAGAAAATTACGAGAAGCAACAGAAACCGGCTA AAAATGGATACAACAATGTTCCACTTCTTCTCAGAAATGTTATCCGTACA GATGCGGATGGAATCAATGGAATTGTACATAGAGTTCGATCTAAATAGgc ggccgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHSPKELGMEEEDVIEVYQE
QTGGEWPNRIDSYEFGEFLGQGCNAAVYSARLANSDAESSGNTHYGAGF
NEVTNILAEIPPVSKVAQKKFPLAIKLMFNFEHDRDGDAHLLKSMGNELA
PYPNAAKLLNGQMGTFRPLPAKHPNVVRIQTAFIDSLKVLPDAIERYPDA
LHTARWYESIASEPKTMYVVMRRYRQTLHEYVWTRHRNYWTGRVIIAQLL
EACTYLHKHKVAQRDMKSDNILLEYDFDDEIPQLVVADFGCALACDNWQV
DYESDEVSLGGNAKTKAPEIATAVPGKNVKVNFEMADTWAAGGLSYEVLT
RSNPFYKLLDTATYQESELPALPSRVNFVARDVIFDLLKRDPNERVKPNI
AANALNLSLFRMGEDVKQMMEKCGISQMTTLLAGSSKVLSQKINSRLDKV
MNLITAETIMANLAPHLISRAERQLRATFLSRMNREDIWRSLQYFFPAGV
QLDTPATSSDCLETISSLMSSFSNDSENYEKQQKPAKNGYNNVPLLLRNV
IRTDADGINGIVHRVRSK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46188
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee