Synonyms
HECTH2
Plasmid
pcDNA5 FRT/TO FLAG UBE3C (Internal BamHI killed so UBE3C digestable using BamHI-NotI)
Parent Plasmid
pcDNA5 FRT/TO FLAG
Expressed
FLAG-UBE3C
Genbank
NM_014671.2 GI:187960099 with silent changes t421c, a1686g, c2251a, g2253a
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
GGATCCATGTTCAGCTTCGAAGGCGACTTCAAGACGCGGCCCAAGGTGTCCCTTGGCGGCGCGAGCAGGAAGGAGGAAAAGGCTTCTCTTTTACATCGTACTCAGGAAGAAAGAAGAAAGAGAGAGGAAGAAAGGCGAAGGTTGAAAAATGCAATAATTATCCAGTCATTTATTCGAGGCTATAGAGACAGAAAACAGCAATATTCCATCCAAAGAAGTGCATTTGATCGCTGTGCTACCTTGTCACAGTCCGGGGGCGCTTTTCCCATTGCTAATGGCCCCAACCTTACCCTTTTGGTAAGGCAGCTTCTGTTTTTTTACAAACAAAATGAAGACTCAAAACGTTTGATATGGCTGTATCAGAACTTAATTAAACACAGCTCTCTGTTTGTCAAGCAGTTGGATGGATCTGAGAGACTTACATGCCTATTTCAGATAAAAAGATTGATGAGCCTCTGTTGCAGGTTGCTGCAAAACTGTAATGATGACAGTTTGAATGTTGCACTTCCAATGAGAATGCTTGAAGTATTTTCGTCTGAGAATACTTACTTGCCTGTTTTACAAGATGCTAGCTATGTGGTGTCAGTGATTGAACAAATTTTGCACTACATGATTCACAATGGGTATTATAGGTCTCTATATTTGTTGATTAACAGCAAGCTTCCATCAAGTATTGAATATTCTGATTTATCTCGAGTTCCTATAGCAAAAATTTTGCTAGAGAATGTTCTAAAACCATTGCACTTTACTTACAACTCCTGTCCGGAAGGTGCGAGGCAACAAGTTTTTACAGCCTTCACAGAGGAGTTTCTGGCAGCACCTTTTACAGATCAGATTTTTCATTTCATCATTCCGGCGCTTGCAGATGCGCAGACCGTTTTCCCTTACGAGCCCTTTCTGAATGCACTGTTGTTAATAGAGAGTAGATGTTCAAGAAAGAGTGGTGGAGCACCCTGGCTTTTCTATTTCGTTTTAACTGTTGGCGAAAATTATTTGGGGGCCCTCTCTGAGGAAGGGCTGCTGGTGTATTTGCGGGTGCTGCAGACCTTCCTCTCTCAGTTACCAGTCTCTCCTGCCAGCGCGAGCTGTCACGACTCAGCCAGTGACTCTGAGGAGGAGAGTGAAGAAGCCGACAAGCCCTCAAGCCCGGAGGATGGCAGACTGTCAGTATCATACATAACAGAGGAATGCCTGAAGAAGCTGGACACAAAGCAGCAGACCAACACCCTGCTCAACCTGGTGTGGAGGGACTCTGCGAGCGAGGAGGTCTTCACCACCATGGCCTCCGTCTGCCACACGCTGATGGTGCAGCACCGCATGATGGTACCCAAAGTCAGGCTTCTCTACAGTTTAGCCTTTAATGCCAGGTTTCTGAGACATCTTTGGTTTCTAATATCTTCCATGTCAACACGGATGATCACAGGGTCTATGGTACCGTTGCTTCAGGTGATATCCAGGGGTTCTCCTATGTCTTTTGAAGATTCTAGTCGAATCATCCCACTCTTTTATCTTTTTAGCTCCTTGTTTAGTCATTCACTAATTTCCATACATGATAACGAATTCTTCGGTGATCCCATAGAAGTTGTAGGTCAAAGACAATCATCAATGATGCCTTTTACTTTAGAAGAGCTGATAATGTTGTCTCGATGCCTTCGAGATGCATGCCTGGGGATCATCAAGTTGGCTTATCCGGAAACCAAGCCAGAAGTTCGAGAAGAATATATTACAGCATTTCAGAGTATTGGAGTTACTACTAGCTCTGAAATGCAACAATGCATACAGATGGAACAGAAAAGATGGATTCAGTTATTTAAGGTTATCACCAATCTAGTGAAAATGTTGAAGTCCAGAGACACGAGGAGAAATTTTTGTCCTCCAAACCACTGGCTGTCAGAACAAGAAGATATTAAAGCAGATAAGGTCACTCAGCTCTATGTGCCAGCATCCAGACATGTGTGGAGGTTCCGGCGGATGGGGAGGATAGGCCCGCTGCAGTCCACCCTGGACGTGGGTTTGGAGTCCCCGCCGCTGTCTGTGTCTGAGGAAAGACAGCTTGCTGTCCTGACAGAGTTGCCTTTTGTGGTTCCATTTGAGGAACGAGTAAAGATCTTTCAGAGGTTGATTTATGCAGATAAGCAAGAAGTTCAAGGAGATGGTCCATTTCTGGATGGAATTAATGTCACAATAAGAAGAAATTACATTTATGAAGATGCTTATGACAAACTTTCTCCAGAAAATGAGCCTGATTTGAAAAAGAGAATCCGTGTGCACTTGCTCAATGCCCATGGCCTGGATGAAGCTGGCATTGATGGTGGTGGTATTTTCAGAGAGTTTTTAAATGAACTACTGAAGTCAGGATTTAACCCCAACCAGGGGTTCTTTAAGACTACTAATGAAGGGCTTCTGTACCCCAACCCGGCTGCTCAGATGCTTGTGGGAGATTCTTTTGCCAGACATTACTACTTCCTAGGCAGAATGCTTGGAAAGGCTCTCTATGAGAACATGCTGGTGGAGCTGCCCTTTGCAGGCTTCTTTCTTTCCAAGTTGCTTGGAACCAGTGCCGACGTGGACATTCACCACCTCGCCTCCCTAGACCCTGAGGTGTATAAGAATTTGCTCTTTCTGAAGAGCTACGAAGACGATGTGGAGGAGCTTGGGCTGAACTTCACTGTGGTGAACAATGACCTGGGAGAGGCGCAGGTAGTTGAACTAAAATTCGGTGGGAAAGACATCCCTGTCACCAGCGCCAACCGGATTGCGTACATCCACTTGGTGGCAGACTACAGGCTGAACAGGCAGATCCGCCAGCACTGCCTGGCTTTCCGCCAGGGCCTTGCCAATGTCGTCAGCCTCGAGTGGCTCCGAATGTTTGATCAGCAAGAAATTCAGGTATTAATTTCTGGTGCACAAGTTCCCATAAGCCTAGAGGACCTAAAATCCTTTACAAACTATTCAGGAGGCTATTCTGCAGACCATCCTGTTATTAAGGTCTTCTGGAGAGTTGTGGAAGGGTTCACTGATGAAGAAAAGCGCAAACTGCTGAAGTTTGTAACAAGCTGCTCTCGACCCCCTCTCTTGGGGTTTAAGGAGTTGTATCCCGCATTTTGTATTCACAACGGAGGCTCCGACCTTGAGCGGCTCCCCACAGCCAGCACCTGCATGAACCTGCTGAAGCTCCCCGAGTTCTATGACGAGACACTTTTGCGAAGTAAACTTCTCTATGCGATTGAATGTGCCGCTGGCTTTGAGCTGAGCTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MDYKDDDDKGSMFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKR
EEERRRLKNAIIIQSFIRGYRDRKQQYSIQRSAFDRCATLSQSGGAFPIA
NGPNLTLLVRQLLFFYKQNEDSKRLIWLYQNLIKHSSLFVKQLDGSERLT
CLFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLQDAS
YVVSVIEQILHYMIHNGYYRSLYLLINSKLPSSIEYSDLSRVPIAKILLE
NVLKPLHFTYNSCPEGARQQVFTAFTEEFLAAPFTDQIFHFIIPALADAQ
TVFPYEPFLNALLLIESRCSRKSGGAPWLFYFVLTVGENYLGALSEEGLL
VYLRVLQTFLSQLPVSPASASCHDSASDSEEESEEADKPSSPEDGRLSVS
YITEECLKKLDTKQQTNTLLNLVWRDSASEEVFTTMASVCHTLMVQHRMM
VPKVRLLYSLAFNARFLRHLWFLISSMSTRMITGSMVPLLQVISRGSPMS
FEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTL
EELIMLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTSSEMQQ
CIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKV
TQLYVPASRHVWRFRRMGRIGPLQSTLDVGLESPPLSVSEERQLAVLTEL
PFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDK
LSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQ
GFFKTTNEGLLYPNPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPF
AGFFLSKLLGTSADVDIHHLASLDPEVYKNLLFLKSYEDDVEELGLNFTV
VNNDLGEAQVVELKFGGKDIPVTSANRIAYIHLVADYRLNRQIRQHCLAF
RQGLANVVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSAD
HPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGG
SDLERLPTASTCMNLLKLPEFYDETLLRSKLLYAIECAAGFELS*
EEERRRLKNAIIIQSFIRGYRDRKQQYSIQRSAFDRCATLSQSGGAFPIA
NGPNLTLLVRQLLFFYKQNEDSKRLIWLYQNLIKHSSLFVKQLDGSERLT
CLFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLQDAS
YVVSVIEQILHYMIHNGYYRSLYLLINSKLPSSIEYSDLSRVPIAKILLE
NVLKPLHFTYNSCPEGARQQVFTAFTEEFLAAPFTDQIFHFIIPALADAQ
TVFPYEPFLNALLLIESRCSRKSGGAPWLFYFVLTVGENYLGALSEEGLL
VYLRVLQTFLSQLPVSPASASCHDSASDSEEESEEADKPSSPEDGRLSVS
YITEECLKKLDTKQQTNTLLNLVWRDSASEEVFTTMASVCHTLMVQHRMM
VPKVRLLYSLAFNARFLRHLWFLISSMSTRMITGSMVPLLQVISRGSPMS
FEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTL
EELIMLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTSSEMQQ
CIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKV
TQLYVPASRHVWRFRRMGRIGPLQSTLDVGLESPPLSVSEERQLAVLTEL
PFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDK
LSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQ
GFFKTTNEGLLYPNPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPF
AGFFLSKLLGTSADVDIHHLASLDPEVYKNLLFLKSYEDDVEELGLNFTV
VNNDLGEAQVVELKFGGKDIPVTSANRIAYIHLVADYRLNRQIRQHCLAF
RQGLANVVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSAD
HPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGG
SDLERLPTASTCMNLLKLPEFYDETLLRSKLLYAIECAAGFELS*
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU45962
Unit Source
Location
PPU
MTA
Price
£125.00
To submit an order for this reagent acceptance of the Dundee Material Transfer Agreement is required at checkout.