PINK1 (Acyrthosiphon pisum)

• Plasmid: pET6HIS SUMO pink1 Acyrthosiphon 146-578 + linker
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: His-SUMO-Acyrthosiphon PINK1 kinase-dead [residues 146-end] [D370A]
• Genbank: XM_001946909
• Tag: 6His
• Tag: SUMO
• Species: Acyrthosiphon pisum

Sequence of Insert

ggatccctgaaattaaattcatctttgggtttgaagcagtttgaattagg
atctgttattgctaaaggaaataatgctgttgtatacgaagcaagaaaaa
ttgaaaaggacagagacccaagtgacgtaatcaagcaagggtcatcaaat
acaatgtatcctttggctgtgaaaatgatgtttaattatgatgctgaaag
taatgccatgtcaatattacgtgccatgagtagagaaacggttccatctc
gtagtgtgcaacatgatttttctagatcagatcagcaatggcctattggt
tatgatcaaaatttaattaaattatcaccacacccaaacattgttatgat
gcatttgtgctttgcggattacgtgccagagttgattgattcgcatacat
tatatcctcaagctctaccaactagaataaatccagaaggttatggtcgg
aacatgagtctatttctggtgatgaaaaagtatgacatgagtttgaaaca
gtatttatctaaagctccatcactgaaaattagagataaaattcttctgc
tcgctcaattattggaagctgtttctcatttgtctaatcaaaacgtggca
catagggatatgaagactgataatatcttattagatgtatccgaaggtaa
tgatgtatgtcctgcattagtcattacagattttgggtgttgtcttgctg
ataaggataatggacttaatcttccatataaatctgctgacactgatcgt
ggaggaaatatagcgctgatggcaccagaagttgtcactgctcgaccagg
aacatttggatatatagattattcaaaagcagatgtatgggctgttggtg
ctatggcatatgaaatattttctgataataatccattttatagttatgaa
agaggtaaacctccattgttgcgcaattctacttacaaggaagaagatct
tcctgcatttagtagtgacataccattaattatacaaacattaattcact
cactattgcagagatcaacatctaagagattagatgcatcatttgctgct
acggtttgtcagatatatctatgggcaccatcttcatggattacaaaata
tgtgatgccaactagtaatgaggtgttgcaatggatgttgtgtttaacaa
ccaaggtgttatgtgaaggaggaacaatgatgtcaacttcaagcactaaa
caacgtacctgtacagaatatcaattgatcactacatttttacatagagc
aaaattgtatgaaataaaaaatgcgttatattggatgcattcaatacaac
aagaataagcggccgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSLKLNSSLGLKQFELGSVIAKGNNAVVYEARKIEKDRDPSDVIKQG
SSNTMYPLAVKMMFNYDAESNAMSILRAMSRETVPSRSVQHDFSRSDQQW
PIGYDQNLIKLSPHPNIVMMHLCFADYVPELIDSHTLYPQALPTRINPEG
YGRNMSLFLVMKKYDMSLKQYLSKAPSLKIRDKILLLAQLLEAVSHLSNQ
NVAHRDMKTDNILLDVSEGNDVCPALVITDFGCCLADKDNGLNLPYKSAD
TDRGGNIALMAPEVVTARPGTFGYIDYSKADVWAVGAMAYEIFSDNNPFY
SYERGKPPLLRNSTYKEEDLPAFSSDIPLIIQTLIHSLLQRSTSKRLDAS
FAATVCQIYLWAPSSWITKYVMPTSNEVLQWMLCLTTKVLCEGGTMMSTS
STKQRTCTEYQLITTFLHRAKLYEIKNALYWMHSIQQE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46135
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee