PINK1 (Rat)

• Plasmid: pET6HIS SUMO pink1 rat 144-580 + linker
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: His-SUMO-Rat PINK1 [residues 144-end]
• Genbank: B5DFG1
• Tag: 6His
• Tag: SUMO
• Species: Rat

Sequence of Insert

agatctgacacacgacgttggcagggcttccgcctggaggattatctgat
aggacaggccatcggcaagggctgcaatgccgctgtgtatgaagccacca
tgcccacactgccccagcacctggagaaggccaaacaccttggccttcta
ggaaaaggcccagatgtcgtctcaaagggagcagatggggagcaggctcc
aggggcccccgccttcccctttgccatcaaaatgatgtggaatatctcgg
caggatcctccagcgaagccatcttaagcaaaatgagccaggagctggtc
ccggcaagccgcatggctttggatggagagtatggagccgttacttacag
aagatccagagacggtcccaagcagcttgccccgcaccccaacatcatcc
gggttttccgcgccttcacttcatctgtgcccctcctgcccggggccctg
gctgactatcctgacatgctacccccacactactacccagaaggcctggg
ccacggccgcacactcttcctcgttatgaagaactacccctgtaccctgc
gccagtaccttgaagagcaaactcccagctcccgcctggctaccatgatg
accttgcagttactggagggcgtggaccacctggttcagcagggcattgc
ccatcgagacctcaagtccgacaacatacttgtggaatgggactcagatg
gctgcccctggctggtgatctccgactttggctgctgcctggctgatgag
cgtgttggcctgcagttacctttcaacagctccagtgtagagcgtggtgg
caatggctccctcatggcccctgaggtgtccacagcccattctggccccc
atgcagtaattgactacagcaaggctgacacgtgggctgtgggggccatc
gcctatgaaatcttcggccttgccaaccccttctatggccaaggcagtgc
ccaccttgagagccgcagctaccaggaagctcagctgcccgagatgccca
agtcggtgcctccagaaacaagacagctggtgaggtccctgctccagcga
gaggccaacaagaggccgtctgcgcgcatagctgccaacgtgctgcactt
aagcctctggggtgagcatcttctagccctgaagaatctgaaattggaca
agatgattgcctggctcctccagcagtcagcagccactctgctggctgac
aggctgagagagaagagctgcgtggagacgaagctgcagatgctgttcct
ggctaacctggagtgtgaggcactctgccaggcagccctcctcctctctt
cctggagggcagccccatgagcggcgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSDTRRWQGFRLEDYLIGQAIGKGCNAAVYEATMPTLPQHLEKAKHL
GLLGKGPDVVSKGADGEQAPGAPAFPFAIKMMWNISAGSSSEAILSKMSQ
ELVPASRMALDGEYGAVTYRRSRDGPKQLAPHPNIIRVFRAFTSSVPLLP
GALADYPDMLPPHYYPEGLGHGRTLFLVMKNYPCTLRQYLEEQTPSSRLA
TMMTLQLLEGVDHLVQQGIAHRDLKSDNILVEWDSDGCPWLVISDFGCCL
ADERVGLQLPFNSSSVERGGNGSLMAPEVSTAHSGPHAVIDYSKADTWAV
GAIAYEIFGLANPFYGQGSAHLESRSYQEAQLPEMPKSVPPETRQLVRSL
LQREANKRPSARIAANVLHLSLWGEHLLALKNLKLDKMIAWLLQQSAATL
LADRLREKSCVETKLQMLFLANLECEALCQAALLLSSWRAAP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46134
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee