PINK1 (Nasonia vitripennis)

• Plasmid: pET6HIS SUMO Pink1 nasonia 153-614 + linker
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO Pink1 nasonia 153-614 + linker
• Genbank: XM_001605929
• Tag: 6His
• Tag: SUMO
• Species: Nasonia vitripennis

Sequence of Insert

ggatctaattatgaaacgaccttaacagatgacaaccaaattagtttgca
aaattttgtaataggaccactgatagcaaaaggctgttcagcagtagtgt
acgcagctaggacaaagaacagcgatgataatcaaaacagtgaagagata
acaattgatagaagcagcaatataacttcttttccattagcaataaaaat
gatgttcaactatgatgctgaatccaatgcaacagcgattctaagagcga
tgttcagggaaacggttcctgcctggaaacattacaaaaatgaggaactg
tcccattgggagcaaaaaatggcagataacaagaaaaccttaccagccca
tagtaatattgtggcgatgtattccgtttttgctgatagagtacctttcc
tgcctggctctataaaaatgtatcccgatgctttgccagcaagacttaat
ccagaaggttctggcagaaatatgagtctttttttgctgatgaaaagata
cgacgttacattgaaacagtatgtcagcgaccgacagcttaacactcgag
aatcgattcttttatttgcacagttgctggaaggtgtagctcacatgaac
tctcacggaattgctcacagggacttgaaagctgacaacatacttcttga
catctcggaagaatccgacaattgtcctacgttagtgatcactgactttg
gatgctgtttggcagacaaaaatcatgggctatatctcccttataacacg
cacgatactgacaagggtggaaacgcagcgctgatggctccagaaattgt
tacggctgagccaggccccttcactagtctcaattatacgaaatcggatc
tttggactgttggtactattgcttatgaaattttcggtatgaaaaatcct
ttttatggaagcgttaaagagaaagctatgcttaaaaactacgactacaa
agtcgaggatcttcctcctctacccgagacggttccagaaataatttcag
ctcttgttagaaatattttaacgcgcagtacctataagcgtcttagtgtg
gagatggcagcgacaataatgcaattgtttctttgggcacccagttcctg
gcttcaaaaagacagtaaaattccatctagcagtgagattttacaatggc
tgttgtgcttaacaactaaagtattgtgcgaagggcgaaatgattctttg
gaagagcgaaacagtatcgatgctcagaaaacagaatcaccgcatcaact
ctcctatcggcgctcgttatccacgcgatcctgcggaaggcgtactatgc
cagaatatcaattgatagcaagctttctgggaagagtaagtttgttcaat
attcgagaaggatttaaatggatccaaagaaacaactatgtctaggcggc
cgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSNYETTLTDDNQISLQNFVIGPLIAKGCSAVVYAARTKNSDDNQNS
EEITIDRSSNITSFPLAIKMMFNYDAESNATAILRAMFRETVPAWKHYKN
EELSHWEQKMADNKKTLPAHSNIVAMYSVFADRVPFLPGSIKMYPDALPA
RLNPEGSGRNMSLFLLMKRYDVTLKQYVSDRQLNTRESILLFAQLLEGVA
HMNSHGIAHRDLKADNILLDISEESDNCPTLVITDFGCCLADKNHGLYLP
YNTHDTDKGGNAALMAPEIVTAEPGPFTSLNYTKSDLWTVGTIAYEIFGM
KNPFYGSVKEKAMLKNYDYKVEDLPPLPETVPEIISALVRNILTRSTYKR
LSVEMAATIMQLFLWAPSSWLQKDSKIPSSSEILQWLLCLTTKVLCEGRN
DSLEERNSIDAQKTESPHQLSYRRSLSTRSCGRRTMPEYQLIASFLGRVS
LFNIREGFKWIQRNNYV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46133
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee