PINK1 (Anopheles gambiae)

• Plasmid: pET6HIS SUMO pink1 anapheles 150-674 D466A
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO pink1 anapheles 150-674 D466A
• Genbank: XP_313587
• Tag: 6His
• Tag: SUMO
• Species: Anopheles gambiae

Sequence of Insert

ggagaatctcgactggacgaagagttcggcattgagaacttaaacattgg
caaacccatcgccaaaggctgctccgccgttgtttacgcagcttctctca
aagaatctaccgtaagcgataacgatgatatgaactgcaatgatattcat
aactcaaagtccagttcgaatgggaaatgtccgaatgaagcatggacagg
agatgagccggagctttctggacagcggagtgacgggattagtccgctgc
tgcttcccgaacgaactgcatcggaggctttccatcaaatgatgaacgaa
agcaatgcgcaaacgagaacaaacaacaatcgtaaagttcgctttaatag
tgaaactagaataagaacaatgtcggaaaacagcgcctccaatcatgaaa
ccattgggcattatcagcctgactcaatcgaacaggactacacgatcgaa
gagtatccactggcgttaaagatgatgtttaattacgacatacaaagcaa
cgccatggctatcctaaaggcgatgtaccgtgaaactgtcccagcgaaga
gaagaactgttgacaacgcgtgggaaaaaagcctcatggaaaagaccaat
tttgttccaccccatccaaatattgtggaaatgtacggcgttttctgcga
ccaggtaccggatttgtcaatgtcggccacgctgtaccctatggcattgc
cacagcggctcaacccgcaaggctacgggcgaaacatgagccttttcctt
ttgatgaaaagatacaacatcaatctgaaagactatctgcgccaaccggg
ggtcgatatgcgtacgcgcattctactgtttgcgcagctgctggaagccg
ttgctcatctgaatcgtcacggggtctctcatcgggatataaagtcggac
aacatactgatcgaattgcggccaaacatgccaccgacgctggtgctgac
cgcctttggctgctgcattgcggacaagcgccatgggctgcgcatccctt
acacttcggacgagatcgataagggtggtaacgtggcactaatggcgccg
gagataatagagcagctaccgggaacgttcgccatgctgaattacaccaa
agccgacctgtgggcatgtggtgcaatcgcttatgaaatatttggcagca
ataatcctttttactccgacgtgaattcggcattaaagaacacgacgtat
gaagaagatatgctacccgcgatggatcaaaatgtcccgaggctgatcca
atgtcttgtgcagaacatactgcaaagaaaccctagtaagcgcttaagcc
cggacattgcagccaacgtggtgcaattgttcttgtggtcaccatcatcg
tggttacgcgataggtacgtaccgtccagcaatgaaattctgcaatggct
gctcagcctcacgacgaaaatactgtgcgaaggcccgttgcgcgtgactc
ccgacggtactatggggcgcagaacgtacacggagtacttactgattgca
agctttcttacccgggtacgcttggaaaggatcaagcgcgcgctcgattg
gatacataatgttaacgctggctgttcgtaagcggccgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGESRLDEEFGIENLNIGKPIAKGCSAVVYAASLKESTVSDNDDMNCN
DIHNSKSSSNGKCPNEAWTGDEPELSGQRSDGISPLLLPERTASEAFHQM
MNESNAQTRTNNNRKVRFNSETRIRTMSENSASNHETIGHYQPDSIEQDY
TIEEYPLALKMMFNYDIQSNAMAILKAMYRETVPAKRRTVDNAWEKSLME
KTNFVPPHPNIVEMYGVFCDQVPDLSMSATLYPMALPQRLNPQGYGRNMS
LFLLMKRYNINLKDYLRQPGVDMRTRILLFAQLLEAVAHLNRHGVSHRDI
KSDNILIELRPNMPPTLVLTAFGCCIADKRHGLRIPYTSDEIDKGGNVAL
MAPEIIEQLPGTFAMLNYTKADLWACGAIAYEIFGSNNPFYSDVNSALKN
TTYEEDMLPAMDQNVPRLIQCLVQNILQRNPSKRLSPDIAANVVQLFLWS
PSSWLRDRYVPSSNEILQWLLSLTTKILCEGPLRVTPDGTMGRRTYTEYL
LIASFLTRVRLERIKRALDWIHNVNAGCS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46131
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee