PINK1 (Anopheles gambiae)

• Plasmid: pET6HIS SUMO pink1 anapheles 150-674 + linker
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO pink1 anapheles 150-674 + linker
• Genbank: XP_313587
• Tag: 6His
• Tag: SUMO
• Species: Anopheles gambiae

Sequence of Insert

ggagaatctcgactggacgaagagttcggcattgagaacttaaacat
tggcaaacccatcgccaaaggctgctccgccgttgtttacgcagcttctc
tcaaagaatctaccgtaagcgataacgatgatatgaactgcaatgatatt
cataactcaaagtccagttcgaatgggaaatgtccgaatgaagcatggac
aggagatgagccggagctttctggacagcggagtgacgggattagtccgc
tgctgcttcccgaacgaactgcatcggaggctttccatcaaatgatgaac
gaaagcaatgcgcaaacgagaacaaacaacaatcgtaaagttcgctttaa
tagtgaaactagaataagaacaatgtcggaaaacagcgcctccaatcatg
aaaccattgggcattatcagcctgactcaatcgaacaggactacacgatc
gaagagtatccactggcgttaaagatgatgtttaattacgacatacaaag
caacgccatggctatcctaaaggcgatgtaccgtgaaactgtcccagcga
agagaagaactgttgacaacgcgtgggaaaaaagcctcatggaaaagacc
aattttgttccaccccatccaaatattgtggaaatgtacggcgttttctg
cgaccaggtaccggatttgtcaatgtcggccacgctgtaccctatggcat
tgccacagcggctcaacccgcaaggctacgggcgaaacatgagccttttc
cttttgatgaaaagatacaacatcaatctgaaagactatctgcgccaacc
gggggtcgatatgcgtacgcgcattctactgtttgcgcagctgctggaag
ccgttgctcatctgaatcgtcacggggtctctcatcgggatataaagtcg
gacaacatactgatcgaattgcggccaaacatgccaccgacgctggtgct
gaccgactttggctgctgcattgcggacaagcgccatgggctgcgcatcc
cttacacttcggacgagatcgataagggtggtaacgtggcactaatggcg
ccggagataatagagcagctaccgggaacgttcgccatgctgaattacac
caaagccgacctgtgggcatgtggtgcaatcgcttatgaaatatttggca
gcaataatcctttttactccgacgtgaattcggcattaaagaacacgacg
tatgaagaagatatgctacccgcgatggatcaaaatgtcccgaggctgat
ccaatgtcttgtgcagaacatactgcaaagaaaccctagtaagcgcttaa
gcccggacattgcagccaacgtggtgcaattgttcttgtggtcaccatca
tcgtggttacgcgataggtacgtaccgtccagcaatgaaattctgcaatg
gctgctcagcctcacgacgaaaatactgtgcgaaggcccgttgcgcgtga
ctcccgacggtactatggggcgcagaacgtacacggagtacttactgatt
gcaagctttcttacccgggtacgcttggaaaggatcaagcgcgcgctcga
ttggatacataatgttaacgctggctgttcgtaagcggccgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGESRLDEQTGGESRLDEQTGGESRLDEEFGIENLNIGKPIAKGCSAV
VYAASLKESTVSDNDDMNCNDIHNSKSSSNGKCPNEAWTGDEPELSGQRS
DGISPLLLPERTASEAFHQMMNESNAQTRTNNNRKVRFNSETRIRTMSEN
SASNHETIGHYQPDSIEQDYTIEEYPLALKMMFNYDIQSNAMAILKAMYR
ETVPAKRRTVDNAWEKSLMEKTNFVPPHPNIVEMYGVFCDQVPDLSMSAT
LYPMALPQRLNPQGYGRNMSLFLLMKRYNINLKDYLRQPGVDMRTRILLF
AQLLEAVAHLNRHGVSHRDIKSDNILIELRPNMPPTLVLTDFGCCIADKR
HGLRIPYTSDEIDKGGNVALMAPEIIEQLPGTFAMLNYTKADLWACGAIA
YEIFGSNNPFYSDVNSALKNTTYEEDMLPAMDQNVPRLIQCLVQNILQRN
PSKRLSPDIAANVVQLFLWSPSSWLRDRYVPSSNEILQWLLSLTTKILCE
GPLRVTPDGTMGRRTYTEYLLIASFLTRVRLERIKRALDWIHNVNAGCS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46103
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee