parkin

• Plasmid: pET6HIS SUMO Parkin Q316A
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: His-SUMO-Parkin [Q316A]
• Genbank: O60260
• Tag: SUMO
• Tag: 6His
• Species: Human

Sequence of Insert

CCATGGGTCATCATCACCATCACCATTCTGACCAGGAGGCAAAACCTTCA
ACTGAGGACTTGGGGGATAAGAAGGAAGGTGAATATATTAAACTCAAAGT
CATTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATGACAACAC
ATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATG
AACTCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATAC
TCCAAAAGAACTGGGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGG
AACAAACGGGGGGAatgatagtgtttgtcaggttcaactccagccatggt
ttcccagtggaggtcgattctgacaccagcatcttccagctcaaggaggt
ggttgctaagcgacagggggttccggctgaccagttgcgtgtgattttcg
cagggaaggagctgaggaatgactggactgtgcagaattgtgacctggat
cagcagagcattgttcacattgtgcagagaccgtggagaaaaggtcaaga
aatgaatgcaactggaggcgacgaccccagaaacgcggcgggaggctgtg
agcgggagccccagagcttgactcgggtggacctcagcagctcagtcctc
ccaggagactctgtggggctggctgtcattctgcacactgacagcaggaa
ggactcaccaccagctggaagtccagcaggtagatcaatctacaacagct
tttatgtgtattgcaaaggcccctgtcaaagagtgcagccgggaaaactc
agggtacagtgcagcacctgcaggcaggcaacgctcaccttgacccaggg
tccatcttgctgggatgatgttttaattccaaaccggatgagtggtgaat
gccaatccccacactgccctgggactagtgcagaatttttctttaaatgt
ggagcacaccccacctctgacaaggaaacatcagtagctttgcacctgat
cgcaacaaatagtcggaacatcacttgcattacgtgcacagacgtcagga
gccccgtcctggttttccagtgcaactcccgccacgtgatttgcttagac
tgtttccacttatactgtgtgacaagactcaatgatcggcagtttgttca
cgaccctcaacttggctactccctgccttgtgtggctggctgtcccaact
ccttgattaaagagctccatcacttcaggattctgggagaagagcagtac
aaccggtacGCgcagtatggtgcagaggagtgtgtcctgcagatgggggg
cgtgttatgcccccgccctggctgtggagcggggctgctgccggagcctg
accagaggaaagtcacctgcgaagggggcaatggcctgggctgtgggttt
gccttctgccgggaatgtaaagaagcgtaccatgaaggggagtgcagtgc
cgtatttgaagcctcaggaacaactactcaggcctacagagtcgatgaaa
gagccgccgagcaggctcgttgggaagcagcctccaaagaaaccatcaag
aaaaccaccaagccctgtccccgctgccatgtaccagtggaaaaaaatgg
aggctgcatgcacatgaagtgtccgcagccccagtgcaggctcgagtggt
gctggaactgtggctgcgagtggaaccgcgtctgcatgggggaccactgg
ttcgacgtgtaggcggccgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMIVFVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFA
GKELRNDWTVQNCDLDQQSIVHIVQRPWRKGQEMNATGGDDPRNAAGGCE
REPQSLTRVDLSSSVLPGDSVGLAVILHTDSRKDSPPAGSPAGRSIYNSF
YVYCKGPCQRVQPGKLRVQCSTCRQATLTLTQGPSCWDDVLIPNRMSGEC
QSPHCPGTSAEFFFKCGAHPTSDKETSVALHLIATNSRNITCITCTDVRS
PVLVFQCNSRHVICLDCFHLYCVTRLNDRQFVHDPQLGYSLPCVAGCPNS
LIKELHHFRILGEEQYNRYAQYGAEECVLQMGGVLCPRPGCGAGLLPEPD
QRKVTCEGGNGLGCGFAFCRECKEAYHEGECSAVFEASGTTTQAYRVDER
AAEQARWEAASKETIKKTTKPCPRCHVPVEKNGGCMHMKCPQPQCRLEWC
WNCGCEWNRVCMGDHWFDV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46037
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee