parkin

• Plasmid: pET6HIS SUMO Parkin 80-465 Q316A
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: His-SUMO-Parkin [residues 80-465] [Q316A]
• Genbank: O60260
• Tag: SUMO
• Tag: 6His
• Species: Human

Sequence of Insert

CCATGGGTCATCATCACCATCACCATTCTGACCAGGAGGCAAAACCTTCA
ACTGAGGACTTGGGGGATAAGAAGGAAGGTGAATATATTAAACTCAAAGT
CATTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATGACAACAC
ATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATG
AACTCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATAC
TCCAAAAGAACTGGGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGG
AACAAACGGGGGGaatgaatgcaactggaggcgacgaccccagaaacgcg
gcgggaggctgtgagcgggagccccagagcttgactcgggtggacctcag
cagctcagtcctcccaggagactctgtggggctggctgtcattctgcaca
ctgacagcaggaaggactcaccaccagctggaagtccagcaggtagatca
atctacaacagcttttatgtgtattgcaaaggcccctgtcaaagagtgca
gccgggaaaactcagggtacagtgcagcacctgcaggcaggcaacgctca
ccttgacccagggtccatcttgctgggatgatgttttaattccaaaccgg
atgagtggtgaatgccaatccccacactgccctgggactagtgcagaatt
tttctttaaatgtggagcacaccccacctctgacaaggaaacatcagtag
ctttgcacctgatcgcaacaaatagtcggaacatcacttgcattacgtgc
acagacgtcaggagccccgtcctggttttccagtgcaactcccgccacgt
gatttgcttagactgtttccacttatactgtgtgacaagactcaatgatc
ggcagtttgttcacgaccctcaacttggctactccctgccttgtgtggct
ggctgtcccaactccttgattaaagagctccatcacttcaggattctggg
agaagagcagtacaaccggtacGCgcagtatggtgcagaggagtgtgtcc
tgcagatggggggcgtgttatgcccccgccctggctgtggagcggggctg
ctgccggagcctgaccagaggaaagtcacctgcgaagggggcaatggcct
gggctgtgggtttgccttctgccgggaatgtaaagaagcgtaccatgaag
gggagtgcagtgccgtatttgaagcctcaggaacaactactcaggcctac
agagtcgatgaaagagccgccgagcaggctcgttgggaagcagcctccaa
agaaaccatcaagaaaaccaccaagccctgtccccgctgccatgtaccag
tggaaaaaaatggaggctgcatgcacatgaagtgtccgcagccccagtgc
aggctcgagtggtgctggaactgtggctgcgagtggaaccgcgtctgcat
gggggaccactggttcgacgtgtaggcggccgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMNATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGLAVILHT
DSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQCSTCRQATLT
LTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAHPTSDKETSVA
LHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCVTRLNDR
QFVHDPQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYAQYGAEECVL
QMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGFAFCRECKEAYHEG
ECSAVFEASGTTTQAYRVDERAAEQARWEAASKETIKKTTKPCPRCHVPV
EKNGGCMHMKCPQPQCRLEWCWNCGCEWNRVCMGDHWFDV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46038
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee