HUWE1

• Plasmid: pET28a HALO TEV HUWE1 R1269-K1690
• Parent Plasmid: pET28a HALO TEV
• Expressed: HALO TEV HUWE1 R1299-K1690
• Genbank: NM_031407.5
• Tag: HALO

Sequence of Insert

ggatcccgaggagaagaggatacagggcaagaggaaggtggctcccgccg
ggaacctcaagtcaaccagcaacaactAcaacagctcatggacatgggct
tcacaagggaacatgcaatggaggcactgttgaacaccagcaccatggag
caggccacagagtaccttttaacccaccctcctccaatcatgggaggagt
tgttcgggatctcagcatgtctgaagaggaccagatgatgagagcaattg
ctatgtctctgggacaggatattccaatggatcaaagggcagagtcacct
gaggaagttgcttgccggaaggaggaagaggaacggaaagctcgggaaaa
gcaggaggaggaagaggctaaatgtctagagaagttccaggatgctgacc
cgttggaacaagatgagctccacactttcacagatactatgttgccaggc
tgcttccaccttcttgatgagctgccagacacagtataccgtgtgtgtga
cctgatcatgacagcaatcaaacgtaatggagcagattatcgtgacatga
ttctgaagcaagtagtcaatcaggtgtgggaagctgctgatgtattgatc
aaagctgctcttcccctgacaacaagtgacacaaaaaccgtgtcagagtg
gataagtcagatggccacactgccccaggcctccaatttggctactagaa
tcttgcttttaacgctactttttgaggagttgaagctaccttgtgcttgg
gtggttgaatcaagtggcatccttaatgtcctaatcaaactcttggaagt
ggttcagccctgcctccaggcagccaaggagcagaaggaagtccagaccc
caaagtggatcacaccagtgttgctcctgattgatttctatgaaaagaca
gccatctcctcaaaaaggagagcccagatgactaagtacctgcaatccaa
cagcaacaactggcgctggtttgatgatcgctctgggcgttggtgtagtt
acagtgcaagcaacaatagcactattgattctgcctggaaatctggagag
acaagcgtgcgattcactgcaggccgaagaagatacacggtccaattcac
tacaatggtgcaggttaatgaggaaacagggaaccgacgccctgtgatgc
tgactctcctcagggtacctcggctgaataaataagcggccgc

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRN
IIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQ
AFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGENL
YFQGGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNT
STMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQR
AESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDT
MLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAA
DVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKL
PCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDF
YEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAW
KSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNK*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU43883
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee