PINK1 (Trichoplax adherens)

• Plasmid: pET6HIS SUMO Pink1 trichoplax 162-558 D358A (Codon optimized)
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: His-SUMO-Trichoplax PINK1 162-558 D358A
• Genbank: XP_002109370.1
• Tag: SUMO
• Species: Trichoplax adhaerens

Sequence of Insert

GGAGACACCACGAATTCATATAAAGTCGGCCCGCGTCTGGGTTCTCGCAG
TTGCTGTTCTGCGGTGTACGCCACCAAATGCAGTTGTGGCAAAGAATACG
CGATGAAAATGATGTTCAACTACGGTAACTCTAAAGATGACTTCATCCGC
AAAAACTTCCTGCGTGAATATTGCCTGCTGAGTCCGCTGCCGGTGACGCG
TGTTGATGAAATGGAAAGCTCTAAAAACTCCCAGTCACGCATTCTGGTGC
ACCCGAACATCATCCGTCTGGATAAAGTTATCATCGACGAAATCCAATAC
TGTGATGACGCAATGCATGAATACCCGGATGCTCTGCCGCCGTCGCCGCT
GAATCCGAGCGGTCTGGGTCGTCACCGTACCATGTTCCTGTTCATGAAAA
AATACGACATGACGCTGCGCGAATACGTTCATTCCAAAGGCATTATCCCG
AACGATGAAGCAACCATGCTGCTGCTGCAGCTGCTGCAGGGTGTGGACTA
TCTGAACAAAAATTACATTGCTCACCGTGATCTGAAAAGCGACAATATTC
TGATCGAAGAAGTTGATCCGTATCCGCTGCTGGCGATCACCGcCTTCGGC
GAATGCCTGGATGACGCAGCTCTGAAACTGGTTGTCCCGTACATTAGCAA
CTGTATCGATCGCGGCGGTAATAGTTCCCTGATGGCACCGGAAATTAAAA
ACGCTGAACCGGGTCATAATTCTGTTCTGGATTATCGTCGCAGTGACGTC
TGGGCGATTGGCTGCCTGGCCTTTGAAATCTTCGGTCAGGAAAACCCGTT
TCTGAATACCTTCGATTCATCGAGCTATCAAGAATACGAACTGCCGGAAC
TGCCGTCTCGTGTCCCGCTGATTGTGCGTAAAATCGTTCGCAACCTGCTG
CGTAAAGATTCTAGTAATCGTCTGCCGGCATCCCTGGCTATTGCAGCACT
GTCATTTTGGCGTTGGGGTCCGATCAATTGGCGTGCACTGATGATGTATG
AAACCGGTCTGAGTAACCTGAATGAAGAAGATATTCAGCGTTGGCTGTCG
GAAACGGCCTTCCAGCTGAGCATTCAAATCATGCAGTGTCAAGCGCATGG
TCACAAACTGACCCCGGAACTGCAGGCCAAATACCATTTTCTGGTCTCAA
CGAGTGCCGCTGACATCAAACTGGCTGTGGAATCCCTGTATTGCTAAGCG
GCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGDTTNSYKVGPRLGSRSCCSAVYATKCSCGKEYAMKMMFNYGNSKDD
FIRKNFLREYCLLSPLPVTRVDEMESSKNSQSRILVHPNIIRLDKVIIDE
IQYCDDAMHEYPDALPPSPLNPSGLGRHRTMFLFMKKYDMTLREYVHSKG
IIPNDEATMLLLQLLQGVDYLNKNYIAHRDLKSDNILIEEVDPYPLLAIT
AFGECLDDAALKLVVPYISNCIDRGGNSSLMAPEIKNAEPGHNSVLDYRR
SDVWAIGCLAFEIFGQENPFLNTFDSSSYQEYELPELPSRVPLIVRKIVR
NLLRKDSSNRLPASLAIAALSFWRWGPINWRALMMYETGLSNLNEEDIQR
WLSETAFQLSIQIMQCQAHGHKLTPELQAKYHFLVSTSAADIKLAVESLY
C*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU43826
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee