PINK1 (Trichoplax adherens)

• Plasmid: pET6HIS SUMO Pink1 trichoplax 162-558 (Codon optimized)
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: His-SUMO-Trichoplax PINK1 162-558
• Genbank: XP_002109370.1
• Tag: SUMO
• Species: Trichoplax adhaerens

Sequence of Insert

GGAGACACCACGAATTCATATAAAGTCGGCCCGCGTCTGGGTTCTCGCAG
TTGCTGTTCTGCGGTGTACGCCACCAAATGCAGTTGTGGCAAAGAATACG
CGATGAAAATGATGTTCAACTACGGTAACTCTAAAGATGACTTCATCCGC
AAAAACTTCCTGCGTGAATATTGCCTGCTGAGTCCGCTGCCGGTGACGCG
TGTTGATGAAATGGAAAGCTCTAAAAACTCCCAGTCACGCATTCTGGTGC
ACCCGAACATCATCCGTCTGGATAAAGTTATCATCGACGAAATCCAATAC
TGTGATGACGCAATGCATGAATACCCGGATGCTCTGCCGCCGTCGCCGCT
GAATCCGAGCGGTCTGGGTCGTCACCGTACCATGTTCCTGTTCATGAAAA
AATACGACATGACGCTGCGCGAATACGTTCATTCCAAAGGCATTATCCCG
AACGATGAAGCAACCATGCTGCTGCTGCAGCTGCTGCAGGGTGTGGACTA
TCTGAACAAAAATTACATTGCTCACCGTGATCTGAAAAGCGACAATATTC
TGATCGAAGAAGTTGATCCGTATCCGCTGCTGGCGATCACCGACTTCGGC
GAATGCCTGGATGACGCAGCTCTGAAACTGGTTGTCCCGTACATTAGCAA
CTGTATCGATCGCGGCGGTAATAGTTCCCTGATGGCACCGGAAATTAAAA
ACGCTGAACCGGGTCATAATTCTGTTCTGGATTATCGTCGCAGTGACGTC
TGGGCGATTGGCTGCCTGGCCTTTGAAATCTTCGGTCAGGAAAACCCGTT
TCTGAATACCTTCGATTCATCGAGCTATCAAGAATACGAACTGCCGGAAC
TGCCGTCTCGTGTCCCGCTGATTGTGCGTAAAATCGTTCGCAACCTGCTG
CGTAAAGATTCTAGTAATCGTCTGCCGGCATCCCTGGCTATTGCAGCACT
GTCATTTTGGCGTTGGGGTCCGATCAATTGGCGTGCACTGATGATGTATG
AAACCGGTCTGAGTAACCTGAATGAAGAAGATATTCAGCGTTGGCTGTCG
GAAACGGCCTTCCAGCTGAGCATTCAAATCATGCAGTGTCAAGCGCATGG
TCACAAACTGACCCCGGAACTGCAGGCCAAATACCATTTTCTGGTCTCAA
CGAGTGCCGCTGACATCAAACTGGCTGTGGAATCCCTGTATTGCTAAGCG
GCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGDTTNSYKVGPRLGSRSCCSAVYATKCSCGKEYAMKMMFNYGNSKD
DFIRKNFLREYCLLSPLPVTRVDEMESSKNSQSRILVHPNIIRLDKVIID
EIQYCDDAMHEYPDALPPSPLNPSGLGRHRTMFLFMKKYDMTLREYVHSK
GIIPNDEATMLLLQLLQGVDYLNKNYIAHRDLKSDNILIEEVDPYPLLAI
TDFGECLDDAALKLVVPYISNCIDRGGNSSLMAPEIKNAEPGHNSVLDYR
RSDVWAIGCLAFEIFGQENPFLNTFDSSSYQEYELPELPSRVPLIVRKIV
RNLLRKDSSNRLPASLAIAALSFWRWGPINWRALMMYETGLSNLNEEDIQ
RWLSETAFQLSIQIMQCQAHGHKLTPELQAKYHFLVSTSAADIKLAVESL
YC*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU43802
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee