Pink1

• Plasmid: pcDNA5FRTTO PINK1 FLAG T185E T257E S284E
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: PINK1 FLAG T185E T257E S284E
• Genbank: Q9BXM7
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGCGGTGCGACAGGCGCTGGGCCGCGGCCTGCAGCT
GGGTCGAGCGCTGCTGCTGCGCTTCACGGGCAAGCCCGGCCGGGCCTACG
GCTTGGGGCGGCCGGGCCCGGCGGCGGGCTGTGTCCGCGGGGAGCGTCCA
GGCTGGGCCGCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGGCTCGGGCT
CCCTAACCGTCTCCGCTTCTTCCGCCAGTCGGTGGCCGGGCTGGCGGCGC
GGTTGCAGCGGCAGTTCGTGGTGCGGGCCTGGGGCTGCGCGGGCCCTTGC
GGCCGGGCAGTCTTTCTGGCCTTCGGGCTAGGGCTGGGCCTCATCGAGGA
AAAACAGGCGGAGAGCCGGCGGGCGGTCTCGGCCTGTCAGGAGATCCAGG
CAATTTTTACCCAGAAAAGCAAGCCGGGGCCTGACCCGTTGGACACGAGA
CGCTTGCAGGGCTTTCGGCTGGAGGAGTATCTGATAGGGCAGTCCATTGG
TAAGGGCTGCAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCC
AGAACCTGGAGGTGgaAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCA
GGTACCAGTGCACCAGGAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGC
CTTCCCCTTGGCCATCAAGATGATGTGGAACATCTCGGCAGGTTCCTCCA
GCGAAGCCATCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGA
GTGGCCTTGGCTGGGGAGTATGGAGCAGTCgaaTACAGAAAATCCAAGAG
AGGTCCCAAGCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCG
CCTTCACCTCTgaaGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCT
GATGTGCTGCCCTCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGAC
GCTGTTCCTCGTTATGAAGAACTATCCCTGTACCCTGCGCCAGTACCTTT
GTGTGAACACACCCAGCCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTG
CTGGAAGGCGTGGACCATCTGGTTCAACAGGGCATCGCGCACAGAGACCT
GAAATCCGACAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGC
TGGTGATCGCAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTG
CAGTTGCCCTTCAGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCT
GATGGCCCCAGAGGTGTCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTG
ACTACAGCAAGGCTGATGCCTGGGCAGTGGGAGCCATCGCCTATGAAATC
TTCGGGCTTGTCAATCCCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAG
CCGCAGCTACCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTC
CAGACGTGAGACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAG
AGACCATCTGCCCGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGG
TGAACATATTCTAGCCCTGAAGAATCTGAAGTTAGACAAGATGGTTGGCT
GGCTCCTCCAACAATCGGCCGCCACTTTGTTGGCCAACAGGCTCACAGAG
AAGTGTTGTGTGGAAACAAAAATGAAGATGCTCTTTCTGGCTAACCTGGA
GTGTGAAACGCTCTGCCAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAG
CCCTGGACTACAAGGACGACGATGACAAGTAGCGGCCGC

Amino Acid Sequence

MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAA
GPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAV
FLAFGLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQG
FRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVEKSTGLLPGRGPGTSA
PGEGQERAPGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALA
GEYGAVEYRKSKRGPKQLAPHPNIIRVLRAFTSEVPLLPGALVDYPDVLP
SRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGV
DHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPF
SSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLV
NPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSA
RVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCV
ETKMKMLFLANLECETLCQAALLLCSWRAALDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU43716
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee