Pink1 (tribolium)

• Plasmid: pET6HIS SUMO Pink1 tribolium 152-570 D359A
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO Pink1 tribolium 152-570 D359A
• Genbank: XP_968367
• Tag: 6His
• Tag: SUMO
• Species: Tribolium castaneum

Sequence of Insert

TTTGAATCTAACCCGATCACCCTGAATGATCTGAGTCTGGGTAAACCGAT
CGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAGATGACG
AAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTCAACTAC
GATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCGTGAAAC
CGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACAATTGGGAAA
TTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAATATTGTG
GCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGGTTCTAA
AGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAGGTGAAG
GTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGTAATCTG
CAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCTGCTGCT
GGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTATTGCAC
ATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAACCGGAA
TCGCCGATTCTGGTTATCTCGGcTTTTGGCTGCTGTCTGGCGGACAAAAC
CAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAGGCGGTA
ATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGCACCTTT
AGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGCAATCGC
TTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTCGCCTGA
AAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGACGAAGTG
CCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAACCCGAA
TAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGTTTCTGT
GGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACGTCAGGT
GAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTGCGAAGG
CAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTAATTGGCGTC
GCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGCGCGAAA
CTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCTGCCGGA
ACTGGATTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKM
MFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPPH
PNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKRY
DCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDT
SEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQK
PGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKL
PDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLKV
PTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISSFL
CRAKLANVRNALHWIQENLPELD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU43669
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee