Pink1 (Tribolium)

• Plasmid: pET6HIS SUMO Pink1 tribolium 147-570 D359A
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO Pink1 tribolium 147-570 D359A
• Genbank: XP_968367
• Tag: 6His
• Tag: SUMO
• Species: Tribolium castaneum

Sequence of Insert

ATCGACGAAAGCCGCTTTGAATCTAACCCGATCACCCTGAATGATCTGAG
TCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAA
AAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAA
ATGATGTTCAACTACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGC
TATGTATCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACC
TGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCG
CACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGA
ACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGC
ATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGT
TATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCAC
CTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGG
CCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGAT
ACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGcTTTTGGCTGCTG
TCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAA
TGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAG
AAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGC
GGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACG
GCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAA
CTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCT
GAAACGTAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCT
GCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAA
GTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAA
AGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCA
CCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTC
CTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCA
AGAAAACCTGCCGGAACTGGATTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGIDESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYP
FALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRK
HLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFL
LMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDN
LLLDTSEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPE
IICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEG
DLPKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLK
PGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLL
ISSFLCRAKLANVRNALHWIQENLPELD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU43665
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee