Pink1 (Tribolium)

• Plasmid: pET6HIS SUMO Pink1 Tribolium 146-570 D359A
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: His-SUMO-TcPINK1 146-570 D359A
• Genbank: XP_968367
• Tag: 6His
• Tag: SUMO
• Species: Tribolium castaneum

Sequence of Insert

GATATCGACGAAAGCCGCTTTGAATCTAACCCGATCACCCTGAATGATCT
GAGTCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCG
CAAAAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCCCTG
AAAATGATGTTCAACTACGATATTCAGAGCAATTCTATGGAAATCCTGAA
AGCTATGTATCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACG
ACCTGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCG
CCGCACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCA
AGAACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTC
TGCATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAA
CGTTATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCG
CACCTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGA
CGGCCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTG
GATACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGcTTTTGGCTG
CTGTCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACG
AAATGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGC
CAGAAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTG
GGCGGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCT
ACGGCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCG
AAACTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCT
GCTGAAACGTAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATG
TCTGCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTG
AAAGTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCAC
GAAAGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAAT
TCACCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCC
TTCCTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGAT
TCAAGAAAACCTGCCGGAACTGGATTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGDIDESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKY
PFALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRR
KHLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLF
LLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSD
NLLLDTSEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAP
EIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKE
GDLPKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWL
KPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYL
LISSFLCRAKLANVRNALHWIQENLPELD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU43664
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee