Pink1 (tribolium)

• Plasmid: pET6HIS SUMO PINK1 tribolium 145-570 D359A
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: His-SUMO-TcPINK1 145-570 D359A
• Genbank: XP_968367
• Tag: 6His
• Tag: SUMO
• Species: Tribolium castaneum

Sequence of Insert

TACGATATCGACGAAAGCCGCTTTGAATCTAACCCGATCACCCTGAATGA
TCTGAGTCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACT
CCGCAAAAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCC
CTGAAAATGATGTTCAACTACGATATTCAGAGCAATTCTATGGAAATCCT
GAAAGCTATGTATCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACC
ACGACCTGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTG
CCGCCGCACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGAT
CCAAGAACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGC
GTCTGCATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATG
AAACGTTATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTAC
CCGCACCTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATA
TGACGGCCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTG
CTGGATACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGcTTTTGG
CTGCTGTCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTT
ACGAAATGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATC
TGCCAGAAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCT
GTGGGCGGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGT
TCTACGGCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTG
CCGAAACTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAA
CCTGCTGAAACGTAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCA
ATGTCTGCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGT
CTGAAAGTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGAC
CACGAAAGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAA
AATTCACCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGT
TCCTTCCTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTG
GATTCAAGAAAACCTGCCGGAACTGGATTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGYDIDESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNK
YPFALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANR
RKHLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSL
FLLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKS
DNLLLDTSEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMA
PEIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYK
EGDLPKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTW
LKPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEY
LLISSFLCRAKLANVRNALHWIQENLPELD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU43663
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee