NleL

• Synonyms: -
• Plasmid: pGEX6P NleL S170-R782(end)
• Parent Plasmid: pGEX6P1
• Expressed: GST NleL S170-R782(end)
• Genbank: Codon optimised from NC_002695 REGION: 1579163..1581511
• Tag: GST

Sequence of Insert

GGATCCAGCCAGGGCCGCGCGTGCCTGAGCAAAGCGGAACTGACCGCGGATCTGATTTGGCTGAGCGCGAACCGCACCGGCGAAGAAAGCGCGGAAGAACTGAACTATAGCGGCTGCGATCTGAGCGGCCTGAGCCTGGTGGGCCTGAACCTGAGCAGCGTGAACTTTAGCGGCGCGGTGCTGGATGATACCGATCTGCGCATGAGCGATCTGAGCCAGGCGGTGCTGGAAAACTGCAGCTTTAAAAACAGCATTCTGAACGAATGCAACTTTTGCTATGCGAACCTGAGCAACTGCATTATTCGCGCGCTGTTTGAAAACAGCAACTTTAGCAACAGCAACCTGAAAAACGCGAGCTTTAAAGGCAGCAGCTATATTCAGTATCCGCCGATTCTGAACGAAGCGGATCTGACCGGCGCGATTATTATTCCGGGCATGGTGCTGAGCGGCGCGATTCTGGGCGATGTGAAAGAACTGTTTAGCGAAAAAAGCAACACCATTAACCTGGGCGGCTGCTATATTGATCTGAGCGATATTCAGGAAAACATTCTGAGCGTGCTGGATAACTATACCAAAAGCAACAAAAGCATTCTGCTGACCATGAACACCAGCGATGATAAATATAACCATGATAAAGTGCGCGCGGCGGAAGAACTGATTAAAAAAATTAGCCTGGATGAACTGGCGGCGTTTCGCCCGTATGTGAAAATGAGCCTGGCGGATAGCTTTAGCATTCATCCGTATCTGAACAACGCGAACATTCAGCAGTGGCTGGAACCGATTTGCGATGATTTTTTTGATACCATTATGAGCTGGTTTAACAACAGCATTATGATGTATATGGAAAACGGCAGCCTGCTGCAGGCGGGCATGTATTTTGAACGCCATCCGGGCGCGATGGTGAGCTATAACAGCAGCTTTATTCAGATTGTGATGAACGGCAGCCGCCGCGATGGCATGCAGGAACGCTTTCGCGAACTGTATGAAGTGTATCTGAAAAACGAAAAAGTGTATCCGGTGACCCAGCAGAGCGATTTTGGCCTGTGCGATGGCAGCGGCAAACCGGATTGGGATGATGATAGCGATCTGGCGTATAACTGGGTGCTGCTGAGCAGCCAGGATGATGGCATGGCGATGATGTGCAGCCTGAGCCATATGGTGGATATGCTGAGCCCGAACACCAGCACCAACTGGATGAGCTTTTTTCTGTATAAAGATGGCGAAGTGCAGAACACCTTTGGCTATAGCCTGAGCAACCTGTTTAGCGAAAGCTTTCCGATTTTTAGCATTCCGTATCATAAAGCGTTTAGCCAGAACTTTGTGAGCGGCATTCTGGATATTCTGATTAGCGATAACGAACTGAAAGAACGCTTTATTGAAGCGCTGAACAGCAACAAAAGCGATTATAAAATGATTGCGGATGATCAGCAGCGCAAACTGGCGTGCGTGTGGAACCCGTTTCTGGATGGCTGGGAACTGAACGCGCAGCATGTGGATATGATTATGGGCAGCCATGTGCTGAAAGATATGCCGCTGCGCAAACAGGCGGAAATTCTGTTTTGCCTGGGCGGCGTGTTTTGCAAATATAGCAGCAGCGATATGTTTGGCACCGAATATGATAGCCCGGAAATTCTGCGCCGCTATGCGAACGGCCTGATTGAACAGGCGTATAAAACCGATCCGCAGGTGTTTGGCAGCGTGTATTATTATAACGATATTCTGGATCGCCTGCAGGGCCGCAACAACGTGTTTACCTGCACCGCGGTGCTGACCGATATGCTGACCGAACATGCGAAAGAAAGCTTTCCGGAAATTTTTAGCCTGTATTATCCGGTGGCGTGGCGCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSQGRACLSKAELTADLIWL
SANRTGEESAEELNYSGCDLSGLSLVGLNLSSVNFSGAVLDDTDLRMSDL
SQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSNFSNSNLKNASFK
GSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTINLGG
CYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIK
KISLDELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMS
WFNNSIMMYMENGSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQ
ERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSDLAYNWVLLS
SQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGYSLSNLF
SESFPIFSIPYHKAFSQNFVSGILDILISDNELKERFIEALNSNKSDYKM
IADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFC
LGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYN
DILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P NleL S170-R782(end)217.63 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU45299
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee