PINK1 (C.elegans)

• Plasmid: pMal4c PINK1 (C.elegans) mod
• Parent Plasmid: pMAL4C
• Expressed: MBP-C.elegans PINK1
• Genbank: NP_495017
• Tag: MBP
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGTCTATGAAACGATTCGGAAAAGCAGCATATCGAATCGCAAATGAGTTAGTTGCAAAAGGTGGACGACTACCAATTTTCCAACGCTTCCTGCCGAGAATATTTCCCGCCACTTATAATTTAGGAGTTCATGTCGTACTCAAAAAGGCTCCATTTCCACGACAAAATGCTCTACGAATTGCTCGCCTTGTAACTCGCCACGGTCGAGTTTTCCGGCCATTTTCCTCAGTAATAATCGAAAGACATCGATTTCAAAATCAAAATGATTGGCGTCGAAAGTTTCAACCGATTCGTAAAGAATTGCCAAGAAATGTGGATTTAGTCGAACGAATCAGGCAGATATTTGGCAATTCTCTACGATACAATGAGGATTTGAAAAGCACTGAATGGCCGAATAGAATTGATTCTTATGAGTTTGGGGAATTTCTCGGTCAAGGATGCAATGCAGCAGTTTACTCTGCGAGATTAGCCAATTCTGATGCAGAATCCTCAGGGAATACTCACTATGGTGCAGGGTTTAATGAAGTCACAAATATACTTGCAGAAATTCCGCCAGTTAGCAAAGTTGCACAAAAGAAATTCCCGTTGGCAATCAAATTAATGTTTAATTTTGAACATGATCGCGATGGAGATGCTCATCTCTTGAAATCAATGGGAAATGAATTGGCTCCATATCCGAATGCTGCAAAGTTGCTCAATGGACAAATGGGAACATTTAGACCTCTTCCAGCAAAACATCCAAATGTTGTTCGAATTCAGACAGCTTTTATTGATTCGTTAAAAGTTTTGCCAGATGCGATTGAACGATATCCAGATGCCCTTCACACTGCACGTTGGTATGAGTCAATTGCCTCCGAACCGAAAACAATGTACGTAGTAATGAGACGATACCGACAAACACTTCATGAATATGTATGGACTCGTCATCGAAATTATTGGACAGGACGAGTGATAATTGCTCAACTATTAGAAGCATGTACATATCTTCATAAGCATAAAGTTGCTCAGCGAGACATGAAAAGTGATAATATTCTTCTGGAATATGATTTTGACGACGAGATTCCCCAATTAGTTGTCGCCGATTTTGGATGTGCACTTGCATGTGACAATTGGCAAGTAGACTATGAATCAGATGAAGTTAGTCTTGGAGGAAATGCCAAGACAAAAGCACCAGAAATTGCGACGGCTGTTCCTGGAAAGAATGTAAAAGTAAACTTCGAAATGGCAGATACATGGGCAGCTGGAGGCCTTTCTTATGAAGTTCTAACACGATCAAATCCATTCTACAAACTTCTTGATACTGCAACATACCAGGAATCAGAACTACCAGCACTCCCATCTCGTGTCAATTTTGTGGCACGAGATGTCATTTTTGACCTACTCAAGCGAGATCCTAATGAAAGAGTCAAGCCGAATATTGCTGCAAATGCGTTGAATTTGTCATTGTTCAGAATGGGAGAAGATGTGAAGCAGATGATGGAAAAATGTGGAATATCTCAAATGACTACTCTATTGGCTGGAAGTTCTAAAGTTTTGAGTCAAAAAATCAATAGTCGTCTGGACAAAGTGATGAATCTGATTACTGCTGAAACTATCATGGCCAACCTAGCTCCACATTTGATTAGTCGAGCAGAACGACAACTTCGAGCAACATTTCTTTCAAGAATGAATCGAGAAGATATTTGGAGAAGTCTTCAATATTTCTTCCCAGCTGGTGTTCAACTTGACACACCTGCCACATCATCAGACTGTTTGGAGACTATTTCCAGTTTGATGTCGAGTTTTTCAAATGATTCAGAAAATTACGAGAAGCAACAGAAACCGGCTAAAAATGGATACAACAATGTTCCACTTCTTCTCAGAAATGTTATCCGTACAGATGCGGATGGAATCAATGGAATTGTACATAGAGTTCGATCTAAATAGGCGGCCGC

Amino Acid Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGPGSMSMKRFGKAAYRIANELVAKGGRLPIFQRFLPRIFPATYNLGVHVVLKKAPFPRQNALRIARLVTRHGRVFRPFSSVIIERHRFQNQNDWRRKFQPIRKELPRNVDLVERIRQIFGNSLRYNEDLKSTEWPNRIDSYEFGEFLGQGCNAAVYSARLANSDAESSGNTHYGAGFNEVTNILAEIPPVSKVAQKKFPLAIKLMFNFEHDRDGDAHLLKSMGNELAPYPNAAKLLNGQMGTFRPLPAKHPNVVRIQTAFIDSLKVLPDAIERYPDALHTARWYESIASEPKTMYVVMRRYRQTLHEYVWTRHRNYWTGRVIIAQLLEACTYLHKHKVAQRDMKSDNILLEYDFDDEIPQLVVADFGCALACDNWQVDYESDEVSLGGNAKTKAPEIATAVPGKNVKVNFEMADTWAAGGLSYEVLTRSNPFYKLLDTATYQESELPALPSRVNFVARDVIFDLLKRDPNERVKPNIAANALNLSLFRMGEDVKQMMEKCGISQMTTLLAGSSKVLSQKINSRLDKVMNLITAETIMANLAPHLISRAERQLRATFLSRMNREDIWRSLQYFFPAGVQLDTPATSSDCLETISSLMSSFSNDSENYEKQQKPAKNGYNNVPLLLRNVIRTDADGINGIVHRVRSKAAMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTR

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU38819
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee