PINK1 (Tribolium) Codon optimised sequence

• Synonyms: BRPK, FLJ27236, PARK6
• Plasmid: pMal4c PINK1t W465X
• Parent Plasmid: pMAL4C
• Expressed: MBP-PINK1t W465X
• Genbank: NM_
• Tag: MBP
• Species: Tribolium castaneum

Sequence of Insert

GGATCCATGAGCGTCCGTGCAGTTGGCAGCCGCCTGTTCAAACATGGCCGTAGCCTGATCCAGCAGTTCTGTAAACGTGACCTGAATACCACCATTGGCGATAAAATCAATGCCGTGAGCCAGGCAACCGCCGCACCGAGCTCTCTGCCGAAAACCCAAATTCCGAAAAACTTTGCCCTGCGTAATGTCGGCGTGCAGCTGGGTCTGCAAGCACGTCGCATTCTGATCGATAACGTCCTGAATCGTGTGACCAATTCACTGTCGGCCGAACTGCGCAAAAAAGCAACGCGTCGCATCCTGTTCGGCGACAGCGCACCGTTTTTCGCTCTGGTTGGTGTCAGTATTGCCTCCGGCACCGGTATCCTGACGAAAGAAGAAGAACTGGAAGGCGTTTGCTGGGAAATCCGTGAAGCGATCTCTAAAATCAAATGGCAGTACTACGATATCGACGAAAGCCGCTTTGAATCTAACCCGATCACCCTGAATGATCTGAGTCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTCAACTACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGATTTTGGCTGCTGTCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAATGAAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCTGCCGGAACTGGATTAAGCGGCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGPGSMSVRAVGSRLFKHGRSLIQQFCKRDLNTTIGDKINAVSQATAAPSSLPKTQIPKNFALRNVGVQLGLQARRILIDNVLNRVTNSLSAELRKKATRRILFGDSAPFFALVGVSIASGTGILTKEEELEGVCWEIREAISKIKWQYYDIDESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDTSEPESPILVISDFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKLPDEVPTVIQALVANLLK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU37723
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee