RNF4

• Plasmid: pLou3-RNF4-GSG-Ring
• Parent Plasmid: pLOU3
• Expressed: 6His-MBP-RNF4-GSG-Ring
• Genbank: NP_062055.1
• Tag: MBP
• Species: Rat

Sequence of Insert

CCATGGATATGAGTACAAGAAATCCTCAAAGAAAGCGGCGTGGTGGAGCTGTGAATTCTAGACAAACCCAGAAGCGAACTCGGGAAACAACTTCAACCCCTGAGATTTCCTTGGAAGCAGAACCCATAGAACTTGTGGAAACCGTTGGAGATGAAATCGTGGACCTCACCTGTGAATCTTTAGAGCCTGTGGTTGTGGACCTGACTCACAATGACTCTGTTGTGATTGTTGAAGAAAGGAGAAGGCCAAGGAGAAATGGGAGGAGGTTGCGCCAAGACCATGCTGACAGCTGTGTGGTGAGCAGTGATGATGAAGAACTGTCTAAGGACAAAGATGTATACGTGACTACCCATACTCCTCGAAGCACCAAGGATGAAGGAACTACTGGACTTAGGCCCTCGGGCACCGTCAGTTGTCCGATCTGCATGGATGGATACTCTGAGATTGTGCAGAATGGGCGTCTCATTGTTTCTACAGAATGTGGCCACGTCTTCTGTAGCCAGTGCCTCCGTGATTCCCTTAAGAATGCTAACACTTGCCCAACTTGTAGGAAAAAGATCAACCATAAACGGTACCATCCCATTTATATAGGATCCGGTTCGGGCACCGTCAGTTGTCCGATCTGCATGGATGGATACTCTGAGATTGTGCAGAATGGGCGTCTCATTGTTTCTACAGAATGTGGCCACGTCTTCTGTAGCCAGTGCCTCCGTGATTCCCTTAAGAATGCTAACACTTGCCCAACTTGTAGGAAAAAGATCAACCATAAACGGTACCATCCCATTTATATATGATAAAAGCTT

Amino Acid Sequence

MHHHHHHHMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGENLYFQGAMDMSTRNPQRKRRGGAVNSRQTQKRTRETTSTPEISLEAEPIELVETVGDEIVDLTCESLEPVVVDLTHNDSVVIVEERRRPRRNGRRLRQDHADSCVVSSDDEELSKDKDVYVTTHTPRSTKDEGTTGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYIGSGSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pLou3-RNF4-GSG-Ring114.12 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU20802
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee