LRRK2

• Plasmid: pFastbac GST LRRK2 H970-E2527(end) G2019S
• Parent Plasmid: pFastbac GST
• Expressed: GST-LRRK2 970-end G2019S
• Genbank: NM_198578.3 GI:171846277 with silent changes c4872a, a4911g, and coding change t4939a (S1647T) (present in other DU LRRK2 clones)
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCATTCAGACAGCATTTCTTCTCTGGCTTCTGAGAGAGAATATAT
TACATCACTAGACCTTTCAGCAAATGAACTAAGAGATATTGATGCCCTAA
GCCAGAAATGCTGTATAAGTGTTCATTTGGAGCATCTTGAAAAGCTGGAG
CTTCACCAGAATGCACTCACGAGCTTTCCACAACAGCTATGTGAAACTCT
GAAGAGTTTGACACATTTGGACTTGCACAGTAATAAATTTACATCATTTC
CTTCTTATTTGTTGAAAATGAGTTGTATTGCTAATCTTGATGTCTCTCGA
AATGACATTGGACCCTCAGTGGTTTTAGATCCTACAGTGAAATGTCCAAC
TCTGAAACAGTTTAACCTGTCATATAACCAGCTGTCTTTTGTACCTGAGA
ACCTCACTGATGTGGTAGAGAAACTGGAGCAGCTCATTTTAGAAGGAAAT
AAAATATCAGGGATATGCTCCCCCTTGAGACTGAAGGAACTGAAGATTTT
AAACCTTAGTAAGAACCACATTTCATCCCTATCAGAGAACTTTCTTGAGG
CTTGTCCTAAAGTGGAGAGTTTCAGTGCCAGAATGAATTTTCTTGCTGCT
ATGCCTTTCTTGCCTCCTTCTATGACAATCCTAAAATTATCTCAGAACAA
ATTTTCCTGTATTCCAGAAGCAATTTTAAATCTTCCACACTTGCGGTCTT
TAGATATGAGCAGCAATGATATTCAGTACCTACCAGGTCCCGCACACTGG
AAATCTTTGAACTTAAGGGAACTCTTATTTAGCCATAATCAGATCAGCAT
CTTGGACTTGAGTGAAAAAGCATATTTATGGTCTAGAGTAGAGAAACTGC
ATCTTTCTCACAATAAACTGAAAGAGATTCCTCCTGAGATTGGCTGTCTT
GAAAATCTGACATCTCTGGATGTCAGTTACAACTTGGAACTAAGATCCTT
TCCCAATGAAATGGGGAAATTAAGCAAAATATGGGATCTTCCTTTGGATG
AACTGCATCTTAACTTTGATTTTAAACATATAGGATGTAAAGCCAAAGAC
ATCATAAGGTTTCTTCAACAGCGATTAAAAAAGGCTGTGCCTTATAACCG
AATGAAACTTATGATTGTGGGAAATACTGGGAGTGGTAAAACCACCTTAT
TGCAGCAATTAATGAAAACCAAGAAATCAGATCTTGGAATGCAAAGTGCC
ACAGTTGGCATAGATGTGAAAGACTGGCCTATCCAAATAAGAGACAAAAG
AAAGAGAGATCTCGTCCTAAATGTGTGGGATTTTGCAGGTCGTGAGGAAT
TCTATAGTACTCATCCCCATTTTATGACGCAGCGAGCATTGTACCTTGCT
GTCTATGACCTCAGCAAGGGACAGGCTGAAGTTGATGCCATGAAGCCTTG
GCTCTTCAATATAAAGGCTCGCGCTTCTTCTTCCCCTGTGATTCTCGTTG
GCACACATTTGGATGTTTCTGATGAGAAGCAACGCAAAGCCTGCATGAGT
AAAATCACCAAGGAACTCCTGAATAAGCGAGGGTTCCCTGCCATACGAGA
TTACCACTTTGTGAATGCCACCGAGGAATCTGATGCTTTGGCAAAACTTC
GGAAAACCATCATAAACGAGAGCCTTAATTTCAAGATCCGAGATCAGCTT
GTTGTTGGACAGCTGATTCCAGACTGCTATGTAGAACTTGAAAAAATCAT
TTTATCGGAGCGTAAAAATGTGCCAATTGAATTTCCCGTAATTGACCGGA
AACGATTATTACAACTAGTGAGAGAAAATCAGCTGCAGTTAGATGAAAAT
GAGCTTCCTCACGCAGTTCACTTTCTAAATGAATCAGGAGTCCTTCTTCA
TTTTCAAGACCCAGCACTGCAGTTAAGTGACTTGTACTTTGTGGAACCCA
AGTGGCTTTGTAAAATCATGGCACAGATTTTGACAGTGAAAGTGGAAGGT
TGTCCAAAACACCCTAAGGGAATTATTTCGCGTAGAGATGTGGAAAAATT
TCTTTCAAAGAAAAGGAAATTTCCAAAGAACTACATGACACAGTATTTTA
AGCTCCTAGAAAAATTCCAGATTGCTTTGCCAATAGGAGAAGAATATTTG
CTGGTTCCAAGCAGTTTGTCTGACCACAGGCCTGTGATAGAGCTTCCCCA
TTGTGAGAACTCTGAAATTATCATCCGACTATATGAAATGCCTTATTTTC
CAATGGGATTTTGGTCAAGATTAATCAATCGATTACTTGAGATTTCACCT
TACATGCTTTCAGGGAGAGAACGAGCACTTCGCCCAAACAGAATGTATTG
GCGACAAGGCATTTACTTAAATTGGTCTCCTGAAGCTTATTGTCTGGTAG
GATCTGAAGTCTTAGACAATCATCCAGAGAGTTTCTTAAAAATTACAGTT
CCTTCTTGTAGAAAAGGCTGTATTCTTTTGGGCCAAGTTGTGGACCACAT
TGATTCTCTCATGGAAGAATGGTTTCCTGGGTTGCTGGAGATTGATATTT
GTGGTGAAGGAGAAACTCTGTTGAAGAAATGGGCATTATATAGTTTTAAT
GATGGTGAAGAACATCAAAAAATCTTACTTGATGACTTGATGAAGAAAGC
AGAGGAAGGAGATCTCTTAGTAAATCCAGATCAACCAAGGCTCACCATTC
CAATATCTCAGATTGCCCCTGACTTGATTTTGGCTGACCTGCCTAGAAAT
ATTATGTTGAATAATGATGAGTTGGAATTTGAACAAGCTCCAGAGTTTCT
CCTAGGTGATGGCAGTTTTGGATCAGTTTACCGAGCAGCCTATGAAGGAG
AAGAAGTGGCTGTGAAGATTTTTAATAAACATACATCACTCAGGCTGTTA
AGACAAGAGCTTGTGGTGCTTTGCCACCTCCACCACCCCAGTTTGATATC
TTTGCTGGCAGCTGGGATTCGTCCCCGGATGTTGGTGATGGAGTTAGCCT
CCAAGGGTTCCTTGGATCGCCTGCTTCAGCAGGACAAAGCCAGCCTCACT
AGAACCCTACAGCACAGGATTGCACTCCACGTAGCTGATGGTTTGAGATA
CCTCCACTCAGCCATGATTATATACCGAGACCTGAAACCCCACAATGTGC
TGCTTTTCACACTGTATCCCAATGCTGCCATCATTGCAAAGATTGCTGAC
TACAGCATTGCTCAGTACTGCTGTAGAATGGGGATAAAAACATCAGAGGG
CACACCAGGGTTTCGTGCACCTGAAGTTGCCAGAGGAAATGTCATTTATA
ACCAACAGGCTGATGTTTATTCATTTGGTTTACTACTCTATGACATTTTG
ACAACTGGAGGTAGAATAGTAGAGGGTTTGAAGTTTCCAAATGAGTTTGA
TGAATTAGAAATACAAGGAAAATTACCTGATCCAGTTAAAGAATATGGTT
GTGCCCCATGGCCTATGGTTGAGAAATTAATTAAACAGTGTTTGAAAGAA
AATCCTCAAGAAAGGCCTACTTCTGCCCAGGTCTTTGACATTTTGAATTC
AGCTGAATTAGTCTGTCTGACGAGACGCATTTTATTACCTAAAAACGTAA
TTGTTGAATGCATGGTTGCTACACATCACAACAGCAGGAATGCAAGCATT
TGGCTGGGCTGTGGGCACACCGACAGAGGACAGCTCTCATTTCTTGACTT
AAATACTGAAGGATACACTTCTGAGGAAGTTGCTGATAGTAGAATATTGT
GCTTAGCCTTGGTGCATCTTCCTGTTGAAAAGGAAAGCTGGATTGTGTCT
GGGACACAGTCTGGTACTCTCCTGGTCATCAATACCGAAGATGGGAAAAA
GAGACATACCCTAGAAAAGATGACTGATTCTGTCACTTGTTTGTATTGCA
ATTCCTTTTCCAAGCAAAGCAAACAAAAAAATTTTCTTTTGGTTGGAACC
GCTGATGGCAAGTTAGCAATTTTTGAAGATAAGACTGTTAAGCTTAAAGG
AGCTGCTCCTTTGAAGATACTAAATATAGGAAATGTCAGTACTCCATTGA
TGTGTTTGAGTGAATCCACAAATTCAACGGAAAGAAATGTAATGTGGGGA
GGATGTGGCACAAAGATTTTCTCCTTTTCTAATGATTTCACCATTCAGAA
ACTCATTGAGACAAGAACAAGCCAACTGTTTTCTTATGCAGCTTTCAGTG
ATTCCAACATCATAACAGTGGTGGTAGACACTGCTCTCTATATTGCTAAG
CAAAATAGCCCTGTTGTGGAAGTGTGGGATAAGAAAACTGAAAAACTCTG
TGGACTAATAGACTGCGTGCACTTTTTAAGGGAGGTAATGGTAAAAGAAA
ACAAGGAATCAAAACACAAAATGTCTTATTCTGGGAGAGTGAAAACCCTC
TGCCTTCAGAAGAACACTGCTCTTTGGATAGGAACTGGAGGAGGCCATAT
TTTACTCCTGGATCTTTCAACTCGTCGACTTATACGTGTAATTTACAACT
TTTGTAATTCGGTCAGAGTCATGATGACAGCACAGCTAGGAAGCCTTAAA
AATGTCATGCTGGTATTGGGCTACAACCGGAAAAATACTGAAGGTACACA
AAAGCAGAAAGAGATACAATCTTGCTTGACCGTTTGGGACATCAATCTTC
CACATGAAGTGCAAAATTTAGAAAAACACATTGAAGTGAGAAAAGAATTA
GCTGAAAAAATGAGACGAACATCTGTTGAGTAAGAGAGAAATAGGCGGCC
GC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMTQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYSIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU17285
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee