NEDD4

• Plasmid: pcDNA5-FRT/TO-HA-1 NEDD4
• Parent Plasmid: pcDNA5 FRT/TO HA1
• Expressed: HA NEDD4
• Genbank: NM_006154.2
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCAACTTGCGCGGTGGAGGTGTTCGGGCTCCTGGAGGACGA GGAAAATTCACGAATTGTGAGAGTAAGAGTTATAGCCGGAATAGGCCTTG CCAAGAAGGATATATTGGGAGCTAGTGATCCTTACGTGAGAGTGACGTTA TATGACCCAATGAATGGAGTTCTTACAAGTGTGCAAACAAAAACCATTAA AAAGAGTTTGAATCCAAAGTGGAATGAAGAAATATTATTCAGAGTTCATC CTCAGCAGCACCGGCTTCTTTTTGAAGTGTTTGACGAAAACCGATTGACA AGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATTACCGAC AGAAAATCCAAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTC ATCCAAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGACTAAAAATG ACTTATTTACCTAAAACCAGTGGCTCAGAAGATGATAATGCAGAACAGGC TGAGGAATTAGAGCCTGGCTGGGTTGTTTTGGACCAACCAGATGCTGCTT GCCATTTGCAGCAACAACAAGAACCTTCTCCTCTACCTCCAGGGTGGGAA GAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGAATCTAG AAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTG AGAATGGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGG CAGATATCCGAGGAAACAGAAAGTGTTGACAACCAAGAGTCTTCCGAGAA CTGGGAAATTATAAGAGAAGATGAAGCCACCATGTATAGCAGCCAGGCCT TCCCATCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTTGCA GAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGTGAGCCA GCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCT ATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCT GGATTACCACCAGGTTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATA TTATGTAGATCACAATTCCAGAACGACTACTTGGACAAAGCCCACTGTAC AGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGCCAGAGTTCTGCAGGC CCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCAGCC ATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATG CACCAAATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACC TGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATC ACTTGATACTTCCAATGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGA GAACTCACACAGATGGAAGAATCTTCTACATAAATCACAATATAAAAAGA ACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGACCAGC AGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAA AGTTGAAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGC CGAGCAACTGTTCTTGAAGACTCTTACCGGAGAATTATGGGTGTCAAGAG AGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTTGATGGTGAAAAGG GATTGGATTATGGAGGAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAG GAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTACGGACAA TTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACC TCTCTTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCAT GGCAAACTGTTGGATGGTTTTTTCATCCGCCCATTTTACAAGATGATGCT TCACAAACCAATAACCCTTCATGATATGGAATCTGTGGATAGTGAATATT ACAATTCCCTAAGATGGATTCTTGAAAATGACCCAACAGAATTGGACCTC AGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACATGAGCT GAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAAT ATATTTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAA ATGGCTGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCTCAT CAAAATTTTTGATGAAAATGAACTAGAGCTTCTTATGTGTGGACTGGGAG ATGTTGATGTGAATGACTGGAGGGAACATACAAAGTATAAAAATGGCTAC AGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTAATGAT GGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTC GGGTGCCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAG TCATTTACAGTTGAACAGTGGGGTACGCCTGAAAAGCTGCCAAGAGCTCA TACCTGTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAGAAT TATGGGATAAACTTCAGATGGCAATTGAAAACACCCAGGGCTTTGATGGA GTTGATTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMATCAVEVFGLLEDEENSRIVRVRVIAGIGLAKKDILG
ASDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLL
FEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKS
RVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQ
EPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQL
QAQRAFTTRRQISEETESVDNQESSENWEIIREDEATMYSSQAFPSPPPS
SNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQP
TLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETS
QLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPF
FIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGR
IFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQND
IPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGV
AREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFI
GRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWI
LENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQ
WRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDW
REHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGF
AELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQM
AIENTQGFDGVD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32548
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee