Recombinant Protein - FES (1 - 822)

Name: FES (1 - 822)
Price per aliquot (100µg): £110.00
Long Name:
FES proto-oncogene, tyrosine kinase
Synonyms:
FPS, Oncogene FES, feline sarcoma virus , c-fes/fps protein, feline sarcoma oncogene, feline sarcoma (Snyder-Theilen) viral (v-fes)/Fujinami avian sarcoma (PRCII) viral (v-fps) oncogene homolog
Catalog Number (DU Number):
DU 33729
Accession:
P07332-1
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Cobalt-Agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 10 mM DTT, 10 mM MgAc
Enzymatic Substrate:
Poly Glu Tyr (4:1) Final concentration: 1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 96, 807.62 daltons
Average Mass: 96, 868.20 daltons
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
6.22
Native Sequence:
Amino acids M1 – R822 (end) of human FES. Residue M29 of the fusion protein is equivalent to M1 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
BgIII and Not1 sites of pFastBac.Htb

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