Recombinant Protein - ALK (1058 - 1620)

Name: ALK (1058 - 1620)
Price per aliquot (100µg): £110.00
Long Name:
ALK receptor tyrosine kinase
Synonyms:
anaplastic lymphoma kinase (Ki-1), anaplastic lymphoma receptor tyrosine kinase , CD246, ALK
Catalog Number (DU Number):
DU 68168
Accession:
NP_004295.2
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 10 mM DTT, 10 mM MgAc
Enzymatic Substrate:
Poly Glu Tyr (4:1) Final concentration: 1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 89, 616.27 daltons
Average Mass: 89, 674.14 daltons
Protein Activity:
Constitutively Active
Purity:
>75 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
6.58
Native Sequence:
Amino acids V1058 – P1620 (end) of human ALK. Residue V235 of the fusion protein is equivalent to V1058 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
EcoR1 and Not1 sites of pFastBac GST

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