Recombinant Protein - eIF2AK4 (1 - 1649)

Name: eIF2AK4 (1 - 1649)
Price per aliquot (100µg): £110.00
Long Name:
eukaryotic translation initiation factor 2 alpha kinase 4
Synonyms:
GCN2, KIAA1338, EIF2AK4
Catalog Number (DU Number):
DU 67392
Accession:
NM_001013703.4
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
RSRSRSRSRSRSRSR residues 204 – 218 of human ASF-1/SF-2
Calculated molecular mass:
Mono-Isotopic Mass: 213, 600.17 daltons
Average Mass: 213, 734.72 daltons
Protein Activity:
Constitutively Active
Purity:
>75 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
5.87
Native Sequence:
Amino acids M1 – F1649 (end) of human eIF2AK4. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 – 228

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