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Recombinant Protein - ALK2 (175 - 509)

Name: ALK2 (175 - 509)
Price per aliquot (100µg): £110.00
Long Name:
activin A receptor type 1
Synonyms:
ACVR1, ACVRLK2, activin A receptor, type I, SKR1, ACVR1A
Catalog Number (DU Number):
DU19351
Accession:
Q04771-1
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Nickel Agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
Casein
Calculated molecular mass:
Mono-Isotopic Mass: 41, 412.69 daltons
Average Mass: 41, 439.28 daltons
Protein Activity:
Constitutively Active
Purity:
>85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
6.73
Native Sequence:
Amino acids V175 – C509 (end) of human ALK2. Residue V30 of fusion protein is equivalent to V175 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
BamH1 and Not1 sites of pFastBAC HTb

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