Recombinant Protein - CLK2 D327A (138 - 499)

Name: CLK2 D327A (138 - 499)
Price per aliquot (100µg): £110.00
Long Name:
CDC like kinase 2
Synonyms:
CDC-like kinase 2, Clk2
Catalog Number (DU Number):
DU31001
Accession:
NM_001294338.2
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
RNRYRDVSPFDHSR Final concentration: 300 uM
Calculated molecular mass:
Mono-Isotopic Mass: 69, 463.18 daltons
Average Mass: 69, 507.71 daltons
Protein Activity:
Kinase/Ligase/DUB Dead
Purity:
80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 °C
Theoretical PI:
6.41
Native Sequence:
Amino acids R138 – R499 (end) of human CLK2. Residue R232 of the fusion protein is equivalent to R138 of the native enzyme. The GST tag is located at residues 1 – 220. The enzyme has a D327A mutation. Residue D327 is equivalent to A421 of the fusion protein.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and Not1 sites of pFastBAC GST

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