Recombinant Protein - FGF-R3 (436 - 806)

Name: FGF-R3 (436 - 806)
Price per aliquot (100µg): £110.00
Long Name:
fibroblast growth factor receptor 3
Synonyms:
FGFR3, achondroplasia, thanatophoric dwarfism, CEK2, JTK4, CD333, ACH
Catalog Number (DU Number):
DU26340
Accession:
NM_000142.4
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
Poly Glu Tyr (4:1) Final concentration: 1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 67, 934.34 daltons
Average Mass: 67, 978.23 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C [Long term stability to be determined]
Theoretical PI:
5.58
Native Sequence:
Amino acids P436 – T806 (end) of human FGFR3. Residue P232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and Not1 sites of pFastBac GST 6P1

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