Name: PreScission Protease (GST and Alfi tagged)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
Calculated molecular mass:
Mono-Isotopic Mass: 48, 138.77 daltons
Average Mass: 48, 169.55 daltons
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 % 2-mercaptoethanol
-80 deg C
Amino acids G1 – Q182 (end) of human Rhinovirus B14.
Residue G229 of the fusion protein is equivalent to G1538 of the native enzyme. The Alfa tag is located at residues 405 – 424. The GST tag is located at residues 1 - 220.
Thrombin (LVPRGS) residues 221 - 226
Kpn1 and Not1 sites of pGEX4T-1