Recombinant Protein - PreScission Protease (GST and Alfi tagged)

Name: PreScission Protease (GST and Alfi tagged)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU67332
Accession:
NP_740524.1
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved

C-Term
Alfa
Uncleaved
Purification Method:
GSH-Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 48, 138.77 daltons
Average Mass: 48, 169.55 daltons
Purity:
>85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 % 2-mercaptoethanol
Storage Temperature:
-80 deg C
Theoretical PI:
6.81
Native Sequence:
Amino acids G1 – Q182 (end) of human Rhinovirus B14. Residue G229 of the fusion protein is equivalent to G1538 of the native enzyme. The Alfa tag is located at residues 405 – 424. The GST tag is located at residues 1 - 220.
Protease Cleavage:
Thrombin (LVPRGS) residues 221 - 226
Cloning Sites:
Kpn1 and Not1 sites of pGEX4T-1

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