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Recombinant Protein - PPM1M (1 - 459)

Name: PPM1M (1 - 459)
Price per aliquot (100µg): £110.00
Long Name:
protein phosphatase, Mg2+/Mn2+ dependent 1M
Synonyms:
protein phosphatase 1M (PP2C domain containing), PP2Ceta, FLJ32332, protein phosphatase 2C eta
Catalog Number (DU Number):
68179
Accession:
NM_144641.4
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved

Uncleaved
Purification Method:
GSH-Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 77, 910.48 daltons
Average Mass: 77, 960.05 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 2 mM MnCl2, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
5.61
Native Sequence:
Amino acids M1 – H459 (end) of human PPM1M. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 229
Cloning Sites:
BamH1 and Not1 sites of pGEX6P1

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