Recombinant Protein - GSK3 alpha (101 - 483) *

Name: GSK3 alpha (101 - 483) *
Price per aliquot (100µg): £110.00
Long Name:
glycogen synthase kinase 3 alpha
Synonyms:
GSK3A
Catalog Number (DU Number):
DU1894
Accession:
NM_019884.3
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved

Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
Phospho GS [YRRAAVPPSPSLSRHSSPHQS(PO4)EDEEE] Final concentration: 30 μM
Calculated molecular mass:
Mono-Isotopic Mass: 46, 358.73 daltons
Average Mass: 46, 388.02 daltons
Protein Activity:
Constitutively Active
Purity:
>85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C
Theoretical PI:
7.63
Native Sequence:
Amino acids M117 – E479 (end) of human PKB gamma. Residue T29 of the fusion protein is equivalent to T101 of the native enzyme. The His6 tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
BamH1 and Not1 sites into pFastBac HTb

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