Recombinant Protein - eIF2AK1 (1 - 630)

Name: eIF2AK1 (1 - 630)
Price per aliquot (100µg): £110.00
Long Name:
eukaryotic translation initiation factor 2 alpha kinase 1
Synonyms:
EIF2AK1, HRI, heme regulated initiation factor 2 alpha kinase, KIAA1369, HCR
Catalog Number (DU Number):
DU67073
Accession:
M_014413.4
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
SRPKtide [RSRSRSRSRSRSRSR] Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 98, 241.96 daltons
Average Mass: 98, 304.00 daltons
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
5.66
Native Sequence:
Amino acids M1 – G630 (end) of human eIF2AK1. Residue M235 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 229
Cloning Sites:
EcoR1 and Not1 sites of pGEX 6P-1

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